Evaluation of lophine derivatives as chemiluminogens by a flow-injection method was written by Nakashima, Kenichiro;Yamasaki, Hiromi;Kuroda, Naotaka;Akiyama, Shuzo. And the article was included in Analytica Chimica Acta in 1995.Quality Control of 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoic acid This article mentions the following:
A rapid and convenient method for the evaluation of lophine (2,4,5-triphenylimidazole) derivatives as to their chemiluminescence efficiency was developed by using a flow-injection technique. Standard solutions of lophine derivatives were injected into a flow line containing a carrier solution (1.5 × 10-4 M cobalt(II) containing 0.2% hydroxylammonium chloride in 75% methanol), which was successively combined with a flow line containing 100 mM hydrogen peroxide in H2O and a flow line containing 1.0M KOH in H2O, and the chemiluminescence generated was monitored. Addition of hydroxylammonium chloride into a carrier solution resulted in a significant increase in chemiluminescence. Calibration graphs for eighteen derivatives were prepared and the relative chemiluminescence intensities were estimated from the slopes of these graphs. Among the derivatives, the compounds substituted by OMe at the 2- and 3-positions, and COOH, COCl, CONHNH2 and CONHNHCO(CH2)4Me at the 4-position on the Ph ring at the 2-position of the imidazole skeleton showed large chemiluminescence intensities which are comparable to that of lophine. The detection limit of lophine was 72 fmol per injection at a signal-to-noise ratio of 2. In the experiment, the researchers used many compounds, for example, 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoic acid (cas: 5496-35-5Quality Control of 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoic acid).
4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoic acid (cas: 5496-35-5) belongs to imidazole derivatives. Among the different heterocyclic compounds, imidazole is better known due to its broad range of chemical and biological properties. Imidazole has become an important synthon in the development of new drugs. Imidazole also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes.Quality Control of 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoic acid
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem