Wu, Xiaolin; Cao, Bo; Aquino, Patricia; Chiu, Tsu-Pei; Chen, Chao; Jiang, Susu; Deng, Zixin; Chen, Shi; Rohs, Remo; Wang, Lianrong; Galagan, James E.; Dedon, Peter C. published the artcile< Epigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria>, Name: 7H-Purin-2-amine, the main research area is phosphorothioate epigenetics transcription regulation Salmonella; ChIP-seq; DNA modification; DNA target selection; epigenetics; restriction-modification.
Phosphorothioate (PT) DNA modifications-in which a nonbonding phosphate oxygen is replaced with sulfur-represent a widespread, horizontally transferred epigenetic system in prokaryotes and have a highly unusual property of occupying only a small fraction of available consensus sequences in a genome. Using Salmonella enterica as a model, we asked a question of fundamental importance: How do the PT-modifying DndA-E proteins select their GPSAAC/GPSTTC targets. Here, we applied innovative anal., sequencing, and computational tools to discover a novel behavior for DNA-binding proteins: The Dnd proteins are “”parked”” at the G6mATC Dam methyltransferase consensus sequence instead of the expected GAAC/GTTC motif, with removal of the 6mA permitting extensive PT modification of GATC sites. This shift in modification sites further revealed a surprising constancy in the d. of PT modifications across the genome. Computational anal. showed that GAAC, GTTC, and GATC share common features of DNA shape, which suggests that PT epigenetics are regulated in a d.-dependent manner partly by DNA shape-driven target selection in the genome.
Proceedings of the National Academy of Sciences of the United States of America published new progress about Animal gene Role: BSU (Biological Study, Unclassified), PRP (Properties), BIOL (Biological Study) (ang43). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Name: 7H-Purin-2-amine.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem