Category: 443-72-1

Li, Zutan; Jiang, Hangjin; Kong, Lingpeng; Chen, Yuanyuan; Lang, Kun; Fan, Xiaodan; Zhang, Liangyun; Cong, Pian published an article in 2021, the title of the article was Deep6mA: A deep learning framework for exploring similar patterns in DNA N6-methyladenine sites across different species.Recommanded Product: 443-72-1 And the article contains the following content:

N6-methyladenine (6mA) is an important DNA modification form associated with a wide range of biol. processes. Identifying accurately 6mA sites on a genomic scale is crucial for under-standing of 6mA’s biol. functions. However, the existing exptl. techniques for detecting 6mA sites are cost-ineffective, which implies the great need of developing new computational methods for this problem. In this paper, we developed, without requiring any prior knowledge of 6mA and manually crafted sequence features, a deep learning framework named Deep6mA to identify DNA 6mA sites, and its performance is superior to other DNA 6mA prediction tools. Specifically, the 5-fold cross-validation on a benchmark dataset of rice gives the sensitivity and specificity of Deep6mA as 92.96% and 95.06%, resp., and the overall prediction accuracy is 94%. Importantly, we find that the sequences with 6mA sites share similar patterns across different species. The model trained with rice data predicts well the 6mA sites of other three species: Arabidopsis thaliana, Fragaria vesca and Rosa chinensis with a prediction accuracy over 90%. In addition, we find that (1) 6mA tends to occur at GAGG motifs, which means the sequence near the 6mA site may be conservative; (2) 6mA is enriched in the TATA box of the promoter, which may be the main source of its regulating downstream gene expression. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: 443-72-1

The Article related to arabidopsis fragaria rosa n6 methyladenine dna, Plant Biochemistry: Photosynthesis (Algae, Bacteria, and Green Plants) and other aspects.Recommanded Product: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2020, Tsukamoto, Yumiko; Tamura, Toshiki; Maeda, Yumi; Miyake, Kensuke; Ato, Manabu published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was N6-methylated adenine on the target sites of mamA from Mycobacterium bovis BCG enhances macrophage activation by CpG DNA in mice. And the article contained the following:

CpG motifs in DNA sequences are recognized by Toll-like receptor 9 and activate immune cells. Bacterial genomic DNA (gDNA) has modified cytosine bases (5-methylcytosine [5 mC]) and modified adenine bases (6-methyladenine [6 mA]). 5 mC inhibits immune activation by CpG DNA; however, it is unclear whether 6 mA inhibits immune activation by CpG DNA. Mycobacterium bovis BCG (BCG) has three adenine methyltransferases (MTases) that act on specific target sequences. In this study, we examined whether the 6 mA at the target sites of adenine MTases affected the immunostimulatory activity of CpG DNA. Our results showed that only 6 mA located at the target sequence of mamA, an adenine MTase from BCG, enhanced interleukin (IL)-12p40 production from murine bone marrow-derived macrophages (BMDMs) stimulated with CpG DNA. Enhancement of IL-12p40 production in BMDMs was also observed when BMDMs were stimulated with CpG DNA ligated to oligodeoxynucleotides (ODNs) harboring 6 mA. Accordingly, we then evaluated whether gDNA from adenine MTase-deficient BCG was less efficient with regard to stimulation of BMDMs. Indeed, gDNA from a mamA-deficient BCG had less ability to activate BMDMs than that from wild-type BCG. We concluded from these results that adenine methylation on ODNs and bacterial gDNA may enhance immune activity induced by CpG DNA. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to mycobacterium cpg dna adenine methylation macrophage, Immunochemistry: Other (Immunity, Immune Suppression, Tolerance, etc.) and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wei, Wei; Liu, Chao; Wang, Meng; Jiang, Wei; Wang, Caihong; Zhang, Shuqun published an article in 2022, the title of the article was Prognostic signature and tumor immune landscape of N7-methylguanosine-related lncRNAs in hepatocellular carcinoma.COA of Formula: C6H7N5 And the article contains the following content:

Despite great advances in the treatment of liver hepatocellular carcinoma (LIHC), such as immunotherapy, the prognosis remains extremely poor, and there is an urgent need to develop novel diagnostic and prognostic markers. Recently, RNA methylation-related long non-coding RNAs (lncRNAs) have been demonstrated to be novel potential biomarkers for tumor diagnosis and prognosis as well as immunotherapy response, such as N6- methyladenine (m6A) and 5-methylcytosine (m5C). N7-Methylguanosine (m7G) is a widespread RNA modification in eukaryotes, but the relationship between m7G-related lncRNAs and prognosis of LIHC patients as well as tumor immunotherapy response is still unknown. In this study, based on the LIHC patients’ clin. and transcriptomic data from TCGA database, a total of 992 m7G-related lncRNAs that co-expressed with 22 m7G regulatory genes were identified using Pearson correlation anal. Univariate regression anal. was used to screen prognostic m7G-related lncRNAs, and the least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression were applied to construct a 9-m7G-related-lncRNA risk model. The m7G-related lncRNA risk model was validated to exhibit good prognostic performance through Kaplan-Meier anal. and ROC anal. Together with the clinicopathol. features, the m7G-related lncRNA risk score was found to be an independent prognostic factor for LIHC. Furthermore, the high-risk group of LIHC patients was unveiled to have a higher tumor mutation burden (TMB), and their tumor microenvironment was more prone to the immunosuppressive state and exhibited a lower response rate to immunotherapy. In addition, 47 anti-cancer drugs were identified to exhibit a difference in drug sensitivity between the high-risk and low-risk groups. Taken together, the m7G-related lncRNA risk model might display potential value in predicting prognosis, immunotherapy response, and drug sensitivity in LIHC patients. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).COA of Formula: C6H7N5

The Article related to n methylguanosine lncrna hepatocellular carcinoma prognosis tumor immunity, lihc, immune response, lncrna, m7g methylation, prognostic model, Immunochemistry: Other (Immunity, Immune Suppression, Tolerance, etc.) and other aspects.COA of Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Rehman, Mobeen Ur; Chong, Kil To published an article in 2020, the title of the article was DNA6mA-MINT: DNA-6mA modification identification neural tool.Recommanded Product: N-Methyl-7H-purin-6-amine And the article contains the following content:

DNA N6-methyladenine (6mA) is part of numerous biol. processes including DNA repair, DNA replication, and DNA transcription. The 6mA modification sites hold a great impact when their biol. function is under consideration. Research in biochem. experiments for this purpose is carried out and they have demonstrated good results. However, they proved not to be a practical solution when accessed under cost and time parameters. This led researchers to develop computational models to fulfill the requirement of modification identification. In consensus, we have developed a computational model recommended by Chou’s 5-steps rule. The Neural Network (NN) model uses convolution layers to extract the high-level features from the encoded binary sequence. These extracted features were given an optimal interpretation by using a Long Short-Term Memory (LSTM) layer. The proposed architecture showed higher performance compared to state-of-the-art techniques. The proposed model is evaluated on Mus musculus, Rice, and “Combined-species” genomes with 5- and 10-fold cross-validation. Further, with access to a user-friendly web server, publicly available can be accessed freely. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to n6 methyladenine genome dna replication convolutional neural network, chou’s 5-steps rule, convolution neural network (cnn), dna n6-methyladenine, long short-term memory (lstm), computational biology and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Takahashi, Yusuke; Shoura, Massa; Fire, Andrew; Morishita, Shinichi published an article.SDS of cas: 443-72-1 The title of the article was Context-dependent DNA polymerization effects can masquerade as DNA modification signals. And the article contained the following:

Single mol. measurements of DNA polymerization kinetics provide a sensitive means to detect both secondary structures in DNA and deviations from primary chem. structure as a result of modified bases. In one approach to such anal., deviations can be inferred by monitoring the behavior of DNA polymerase using single-mol., real-time sequencing with zero-mode waveguide. This approach uses a Single Mol. Real Time (SMRT)-sequencing measurement of time between fluorescence pulse signals from consecutive nucleosides incorporated during DNA replication, called the interpulse duration (IPD). In this paper we present an anal. of loci with high IPDs in two genomes, a bacterial genome (E. coli) and a eukaryotic genome (C. elegans). To distinguish the potential effects of DNA modification on DNA polymerization speed, we paired an anal. of native genomic DNA with whole-genome amplified (WGA) material in which DNA modifications were effectively removed. Adenine modification sites for E. coli are known and we observed the expected IPD shifts at these sites in the native but not WGA samples. For C. elegans, such differences were not observed Instead, we found a number of novel sequence contexts where IPDs were raised relative to the average IPDs for each of the four nucleotides, but for which the raised IPD was present in both native and WGA samples. The latter results argue strongly against DNA modification as the underlying driver for high IPD segments for C. elegans, and provide a framework for separating effects of DNA modification from context-dependent DNA polymerase kinetic patterns inherent in underlying DNA sequence for a complex eukaryotic genome. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to dna polymerization effect masquerade modification signal, dna n6-methyladenine, dna modification, dna polymerization, non-b dna, single-molecule real-time (smrt) sequencing, whole genome amplification and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 30, 2021, Xue, Tian; Zhang, Shengli; Qiao, Huijuan published an article.Recommanded Product: 443-72-1 The title of the article was i6mA-VC: A Multi-Classifier Voting Method for the Computational Identification of DNA N6-methyladenine Sites. And the article contained the following:

Abstract: DNA N6-methyladenine (6 mA), as an essential component of epigenetic modification, cannot be neglected in genetic regulation mechanism. Most of the established machine learning methods have a single dataset. Although some of them have achieved cross-species prediction, their results are not satisfactory. Therefore, we designed a novel statistical model called i6mA-VC to improve the accuracy for 6 mA sites. On the one hand, kmer and binary encoding are applied to extract features, and then gradient boosting decision tree (GBDT) embedded method is applied as the feature selection strategy. After fusing the two optimal features, a voting classifier based on gradient boosting decision tree (GBDT), light gradient boosting machine (LightGBM) and multilayer perceptron classifier (MLPC) is constructed for final classification and prediction. The accuracy of Rice dataset and M.musculus dataset with five-fold cross-validation are 0.888 and 0.967, resp. The cross-species dataset is selected as independent testing dataset, and the accuracy reaches 0.848. Through rigorous experiments, it is demonstrated that the proposed predictor is convincing and applicable. The development of i6mA-VC predictor will become an effective way for the recognition of N6-methyladenine sites, and it will also be beneficial for biol. geneticists to further study gene expression and DNA modification. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: 443-72-1

The Article related to n6methyladenine machine learning dna sequence genetics, dna n6-methyladenine sites, light gradient boosting machine, multilayer perceptron classifier, ring-function-hydrogen-chemical properties, voting and other aspects.Recommanded Product: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Zhang, Zhongwang; Wang, Lidong published an article in 2022, the title of the article was Using Chou’ s 5-steps rule to identify N6-methyladenine sites by ensemble learning combined with multiple feature extraction methods.Formula: C6H7N5 And the article contains the following content:

N6-methyladenine (m6A), a type of modification mostly affecting the downstream biol. functions and determining the levels of gene expression, is mediated by the methylation of adenine in nucleic acids. It is also a key factor for influencing biol. processes and has attracted attention as a target for treating diseases. Here, an ensemble predictor named as TL-Methy, was developed to identify m6A sites across the genome. TL-Methy is a 2-level machine learning method developed by combining the support vector machine model and multiple features extraction methods, including nucleic acid composition, di-nucleotide composition, tri-nucleotide composition, position-specific trinucleotide propensity, Bi-profile Bayes, binary encoding, and accumulated nucleotide frequency. For Homo sapiens, TL-Methy method reached the accuracy of 91.68% on jackknife test and of 92.23% on 10-fold cross validation test; For Mus musculus, TL-Methy method achieved the accuracy of 93.66% on jackknife test and of 97.07% on 10-fold cross validation test; For Saccharomyces cerevisiae, TL-Methy method obtained the accuracy of 81.57% on jackknife test and of 82.54% on 10-fold cross validation test; For rice genome, TL-Methy method achieved the accuracy of 91.87% on jackknife test and of 93.04% on 10-fold cross validation test. The results via these two test approaches demonstrated the robustness and practicality of our TL-Methy model. The TL-Methy model may be as a potential method for m6A site identification.Communicated by Ramaswamy H. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to saccharomyces cerevisiae mus musculus methyladenine jackknife test feature extraction, 10-fold cross validation test, 2-level method, n6-methyladenine, feature extraction, jackknife test, support vector machine and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Frey, Felice; Sandakly, Jawdat; Ghannam, Mirna; Doueiry, Caren; Hugosson, Fredrik; Berlandi, Johannes; Ismail, Joy N.; Gayden, Tenzin; Hasselblatt, Martin; Jabado, Nada; Shirinian, Margret published an article in 2022, the title of the article was Drosophila tet is required for maintaining glial homeostasis in developing and adult fly brains.SDS of cas: 443-72-1 And the article contains the following content:

Ten-eleven translocation (TET) proteins are crucial epigenetic regulators highly conserved in multicellular organisms. TETs’ enzymic function in demethylating 5-Me cytosine in DNA is required for proper development and TETs are frequently mutated in cancer. Recently, Drosophila melanogaster Tet (dTet) was shown to be highly expressed in developing fly brains and discovered to play an important role in brain and muscle development as well as fly behavior. Furthermore, dTet was shown to have different substrate specificity compared with mammals. However, the exact role dTet plays in glial cells and how ectopic TET expression in glial cells contributes to tumorigenesis and glioma is still not clear. Here, we report a novel role for dTet specifically in glial cell organization and number We show that loss of dTet affects the organization of a specific glia population in the optic lobe, the “optic chiasm” glia. Addnl., we find irregularities in axon patterns in the ventral nerve cord (VNC) both, in the midline and longitudinal axons. These morphol. glia and axonal defects were accompanied by locomotor defects in developing larvae escalating to immobility in adult flies. Furthermore, glia homeostasis was disturbed in dTet-deficient brains manifesting in gain of glial cell numbers and increased proliferation. Finally, we establish a Drosophila model to understand the impact of human TET3 in glia and find that ectopic expression of hTET3 in dTet-expressing cells causes glia expansion in larval brains and affects sleep/rest behavior and the circadian clock in adult flies. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to drosophila melanogaster tet glial homeostasis fly brain development, 6ma dna demethylation, drosophila ten-eleven translocation (tet), drosophila brain, brain tumor, human tet drosophila model, optic chiasm glia and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2021, Wang, Keyan; Li, Yiping; Qiang, Tingting; Chen, Jie; Wang, Xiaolong published an article.HPLC of Formula: 443-72-1 The title of the article was Role of epigenetic regulation in myocardial ischemia/reperfusion injury. And the article contained the following:

A review. Nowadays acute myocardial infarction (AMI) is a serious cardiovascular disease threatening the human life and health worldwide. The most effective treatment is to quickly restore coronary blood flow through revascularization. However, timely revascularization may lead to reperfusion injury, thereby reducing the clin. benefits of revascularization. At present, no effective treatment is available for myocardial ischemia/reperfusion injury. Emerging evidence indicates that epigenetic regulation is closely related to the pathogenesis of myocardial ischemia/reperfusion injury, indicating that epigenetics may serve as a novel therapeutic target to ameliorate or prevent ischemia/reperfusion injury. This aimed to briefly summarize the role of histone modification, DNA methylation, noncoding RNAs, and N6-methyladenosine (m6A) methylation in myocardial ischemia/reperfusion injury, with a view to providing new methods and ideas for the research and treatment of myocardial ischemia/reperfusion injury. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to cardiovascular disease, coronary circulation, dna methylation, epigenetics, histones role: thu (therapeutic use), biol (biological study), uses (uses), homo sapiens, human, ischemia-reperfusion injury, myocardial infarction, non-coding rna role: thu (therapeutic use), biol (biological study), uses (uses) and other aspects.HPLC of Formula: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2022, Li, Zhenwei; Xu, Qingqing; Ning, Huangfu; Chen, Xiaomin; Zhu, Jianhua published an article.Application of 443-72-1 The title of the article was Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling. And the article contained the following:

Atherosclerosis (AS) is the main cause of cerebrovascular diseases, and macrophages act important roles during the AS pathol. process through regulating inflammation. Modification of the novel N(6)-methyladenine (m6A) RNA is reported to be associated with AS, but its role in AS is largely unknown. The aim of this study was to investigate the role and mechanism of m6A modification in inflammation triggered by oxidized low-d. lipoprotein (oxLDL) in macrophages during AS. RAW264.7 macrophage cells were stimulated with 40μg/mL ox-LDL, Dot blot, Immunoprecipitation, western blot, Rip and chip experiments were used in our study. We found oxLDL stimulation significantly promoted m6A modification level of mRNA in macrophages and knockdown of Methyltransferase-Like Protein 3 (Mettl3) inhibited oxLDL-induced m6A modification and inflammatory response. Mettl3 promoted oxLDL-induced inflammatory response in macrophages through regulating m6A modification of Signal transducer and activator of transcription 1 (STAT1) mRNA, thereby affecting STAT1 expression and activation. Moreover, oxLDL stimulation enhanced the interaction between Mettl3 and STAT1 protein, promoting STAT1 transcriptional regulation of inflammatory factor expression in macrophages eventually. These results indicate that Mettl3 promotes oxLDL-triggered inflammation through interacting with STAT1 protein and mRNA in RAW264.7 macrophages, suggesting that Mettl3 may be as a potential target for the clin. treatment of AS. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to mettl3 oxidized low density lipoprotein stat1 signaling inflammation atherosclerosis, n6-methyladenosine, atherosclerosis, inflammation, methyltransferase-like protein 3, signal transducer and activator of transcription 1 and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem