Category: 443-72-1

On May 12, 2021, Wang, Qing; Tan, Kaiyue; Wang, Hong; Shang, Jinhua; Wan, Yeqing; Liu, Xiaoqing; Weng, Xiaocheng; Wang, Fuan published an article.Safety of N-Methyl-7H-purin-6-amine The title of the article was Orthogonal demethylase-activated deoxyribozyme for intracellular imaging and gene regulation. And the article contained the following:

The epigenetic modification of nucleic acids represents a versatile approach for achieving high-efficient control over gene expression and transcription and could dramatically expand their biosensing and therapeutic applications. Demethylase-involved removal of N6-methyladenine (m6A) represents one of the vital epigenetic reprogramming events, yet its direct intracellular evaluation and as-guided gene regulation are extremely rare. The endonuclease-mimicking deoxyribozyme (DNAzyme) is a catalytically active DNA that enables the site-specific cleavage of the RNA substrate, and several strategies have imparted the magnificent responsiveness to DNAzyme by using chem. and light stimuli. However, the epigenetic regulation of DNAzyme has remained largely unexplored, leaving a significant gap in responsive DNA nanotechnol. Herein, we reported an epigenetically responsive DNAzyme system through the in vitro selection of an exquisite m6A-caged DNAzyme that could be specifically activated by FTO (fat mass and obesity-associated protein) demethylation for precise intracellular imaging-directed gene regulation. Based on a systematic investigation, the active DNAzyme configuration was potently disrupted by the site-specific incorporation of m6A modification and subsequently restored into the intact DNAzyme structure via the tunable FTO-specific removal of m6A-caging groups under a variety of conditions. This orthogonal demethylase-activated DNAzyme amplifier enables the robust and accurate monitoring of FTO and its inhibitors in live cells. Moreover, the simple demethylase-activated DNAzyme facilitates the assembly of an intelligent self-adaptive gene regulation platform for knocking down demethylase with the ultimate apoptosis of tumor cells. As a straightforward and scarless m6A removal strategy, the demethylase-activated DNAzyme system offers a versatile toolbox for programmable gene regulation in synthetic biol. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to dnazyme rnase regulation n6 methyladenine cage demethylase fto protein, human cell demethylase activated dnazyme programmable gene regulation, Enzymes: Separation-Purification-General Characterization and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2021, Zhang, Xing; Blumenthal, Robert M.; Cheng, Xiaodong published an article.Recommanded Product: N-Methyl-7H-purin-6-amine The title of the article was A Role for N6-Methyladenine in DNA Damage Repair. And the article contained the following:

The leading cause of mutation due to oxidative damage is 8-oxo-2′-deoxyguanosine (8-oxoG) mispairing with adenine (Ade), which can occur in two ways. First, guanine of a G:C DNA base pair can be oxidized. If not repaired in time, DNA polymerases can mispair Ade with 8-oxoG in the template. This 8-oxoG:A can be repaired by enzymes that remove Ade opposite to template 8-oxoG, or 8-oxoG opposite to Cyt. Second, free 8-oxo-dGTP can be misincorporated by DNA polymerases into DNA opposite template Ade. However, there is no known repair activity that removes 8-oxoG opposite to template Ade. We suggest that a major role of N6-methyladenine in mammalian DNA is minimizing incorporation of 8-oxoG opposite to Ade by DNA polymerases following adduct formation. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to methyladenine dna damage repair, 8-oxoguanine, dna adenine methylation, mettl3-mettl14, ythdc1, single-stranded dna, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 15, 2022, Iwasaki, Yuka; Ookuro, Yurino; Iida, Keisuke; Nagasawa, Kazuo; Yoshida, Wataru published an article.Synthetic Route of 443-72-1 The title of the article was Destabilization of DNA and RNA G-quadruplex structures formed by GGA repeat due to N6-methyladenine modification. And the article contained the following:

N6-methyladenine (m6A) is the most abundant RNA modification in eukaryotic RNA. Further, m6A has been identified in the genomic DNA of both eukaryotes and prokaryotes. The G-quadruplex (G4) structure is a non-canonical nucleic acid structure formed by the stacking of G:G:G:G tetrads. In this study, we evaluated the effect of m6A modifications on G4 structures formed by GGA repeat oligonucleotides, d(GGA)8, d(GGA)4, and r(GGA)4. The d(GGA)8 forms an intramol. tetrad:heptad:heptad:tetrad G4 structure, while d(GGA)4 forms a dimerized intermol. tetrad:heptad:heptad:tetrad G4 structure. r(GGA)4 forms a dimerized intermol. tetrad:hexad:hexad:tetrad G4 structure. CD melting anal. demonstrated that (1) m6A modifications destabilized the G4 structure formed by d(GGA)8, (2) m6A modification at A3 disrupted the G4 structure formed by d(GGA)4, and (3) m6A modification at A3 destabilized the G4 structure formed by r(GGA)4. M6A modifications may be involved in controlling G4 structure formation to regulate biol. functions. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to dna rna gquadruplex structure destabilization gga repeat methyladenine modification, g-quadruplex, gga repeat, n(6)-methyladenine, thermal stability, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Yang, Xiao; Ye, Xiucai; Li, Xuehong; Wei, Lesong published an article in 2021, the title of the article was iDNA-MT: identification DNA modification sites in multiple species by using multi-task learning based a neural network tool.Quality Control of N-Methyl-7H-purin-6-amine And the article contains the following content:

DNA N4-methylcytosine (4mC) and N6-methyladenine (6mA) are two important DNA modifications and play crucial roles in a variety of biol. processes. Accurate identification of the modifications is essential to better understand their biol. functions and mechanisms. However, existing methods to identify 4mA or 6mC sites are all single tasks, which demonstrates that they can identify only a certain modification in one species. Therefore, it is desirable to develop a novel computational method to identify the modification sites in multiple species simultaneously. In this study, we proposed a computational method, called iDNA-MT, to identify 4mC sites and 6mA sites in multiple species, resp. The proposed iDNA-MT mainly employed multi-task learning coupled with the bidirectional gated recurrent units (BGRU) to capture the sharing information among different species directly from DNA primary sequences. Exptl. comparative results on two benchmark datasets, containing different species resp., show that either for identifying 4mA or for 6mC site in multiple species, the proposed iDNA-MT outperforms other state-of-the- art single-task methods. The promising results have demonstrated that iDNA-MT has great potential to be a powerful and practically useful tool to accurately identify DNA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to dna modification site multi task learning neural network tool, dna modification, deep learning, feature representation, multi-task learning, neural network, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2021, Joshi, Suraj; Ujaoney, Aman Kumar; Ghosh, Payel; Deobagkar, Deepti D.; Basu, Bhakti published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine and epigenetic immunity of Deinococcus radiodurans. And the article contained the following:

DNA methylation is ubiquitously found in all three domains of life. This epigenetic modification on adenine or cytosine residues serves to regulate gene expression or to defend against invading DNA in bacteria. Here, we report the significance of N6-methyladenine (6mA) to epigenetic immunity in Deinococcus radiodurans. Putative protein encoded by DR_2267 ORF (Dam2DR) contributed 35% of genomic 6mA in D. radiodurans but did not influence gene expression or radiation resistance. Dam2DR was characterized to be a functional S-adenosyl methionine (SAM)-dependent N6-adenine DNA methyltransferase (MTase) but with no endonuclease activity. Adenine methylation from Dam2DR or Dam1DR (N6-adenine MTase encoded by DR_0643) improved DNA uptake during natural transformation. To the contrary, methylation from Escherichia coli N6-adenine MTase (DamEC that methylates adenine in GATC sequence) on donor plasmid drastically reduced DNA uptake in D. radiodurans, even in presence of Dam2DR or Dam1DR methylated adenines. With these results, we conclude that self-type N6-adenine methylation on donor DNA had a protective effect in absence of addnl. foreign methylation, a sep. methylation-dependent Restriction Modification (R-M) system effectively identifies and limits uptake of G6mATC sequence containing donor DNA. This is the first report demonstrating presence of epigenetic immunity in D. radiodurans. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to deinococcus n6 methyladenine epigenetic immunity, dna uptake, dr_2267, deinococcus radiodurans, n6-methyladenine, restriction modification, transformation efficiency, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2021, Chen, Zheng; Liu, Yan; Wang, Hailin published an article.Application of 443-72-1 The title of the article was Conjoint expression and purification strategy for acquiring proteins with ultra-low DNA N6-methyladenine backgrounds in Escherichia coli. And the article contained the following:

DNA N6-methyladenine (6mA), a kind of DNA epigenetic modification, is widespread in eukaryotes and prokaryotes. An enzyme activity study coupled with 6mA detection using ultra-high-performance liquid chromatog.-quadruple mass spectrometry (UHPLC-MS/MS) is commonly applied to investigate 6mA potentially related enzymes in vitro. However, the protein expressed in a common Escherichia coli (E. coli) strain shows an extremely high 6mA background due to minute co-purified bacterial DNA, though it has been purified to remove DNA using multiple strategies. Furthermore, as occupied by DNA with abundant 6mA, the activity of 6mA-related proteins will be influenced seriously. Here, to address this issue, we for the first time construct a derivative of E. coli Rosetta (DE3) via the λRed knockout system specifically for the expression of 6mA-related enzymes. The gene dam encoding the 6mA methyltransferase (MTase) is knocked out in the newly constructed strain named LAMBS (low adenine methylation background strain). Contrasting with E. coli Rosetta (DE3), LAMBS shows an ultra-low 6mA background on the genomic DNA when analyzed by UHPLC-MS/MS. We also demonstrate an integral strategy of protein purification, coupled with the application of LAMBS. As a result, the purified protein expressed in LAMBS exhibits an ultra-low 6mA background comparing with the one expressed in E. coli Rosetta (DE3). Our integral strategy of protein expression and purification will benefit the in vitro investigation and application of 6mA-related proteins from eukaryotes, although these proteins are elusive until now. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to escherichia dna n6 methyladenine protein expression purification, dna n6-methyladenine, e. coli rosetta (de3), uhplc-ms/ms, low background, λred system, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2021, Chaturvedi, Anurag; Cruz Corella, Joaquim; Robbins, Chanz; Loha, Anita; Menin, Laure; Gasilova, Natalia; Masclaux, Frederic G.; Lee, Soon-Jae; Sanders, Ian R. published an article.Formula: C6H7N5 The title of the article was The methylome of the model arbuscular mycorrhizal fungus, Rhizophagus irregularis, shares characteristics with early diverging fungi and Dikarya. And the article contained the following:

Early-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glomeromycotina) evolved a symbiotic lifestyle with 80% of plant species worldwide. Here we show that these fungi exhibit 5mC and 6mA methylation characteristics that jointly set them apart from other fungi. The model AMF, R. irregularis, evolved very high levels of 5mC and greatly reduced levels of 6mA. However, unlike the Dikarya, 6mA in AMF occurs at sym. ApT motifs in genes and is associated with their transcription. 6MA is heterogeneously distributed among nuclei in these coenocytic fungi suggesting functional differences among nuclei. While far fewer genes are regulated by 6mA in the AMF genome than in EDF, most strikingly, 6mA methylation has been specifically retained in genes implicated in components of phosphate regulation; the quintessential hallmark defining this globally important symbiosis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to rhizophagus irregularis arbuscular mycorrhizal fungus characteristic methyldeoxyadenine methylcytosine, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2020, Wang, Hao-Tian; Xiao, Fu-Hui; Li, Gong-Hua; Kong, Qing-Peng published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was Identification of DNA N6-methyladenine sites by integration of sequence features. And the article contained the following:

Abstract: Background: An increasing number of nucleic acid modifications have been profiled with the development of sequencing technologies. DNA N6-methyladenine (6mA), which is a prevalent epigenetic modification, plays important roles in a series of biol. processes. So far, identification of DNA 6mA relies primarily on time-consuming and expensive exptl. approaches. However, in silico methods can be implemented to conduct preliminary screening to save exptl. resources and time, especially given the rapid accumulation of sequencing data. Results: In this study, we constructed a 6mA predictor, p6mA, from a series of sequence-based features, including physicochem. properties, position-specific triple-nucleotide propensity (PSTNP), and electron-ion interaction pseudopotential (EIIP). We performed maximum relevance maximum distance (MRMD) anal. to select key features and used the Extreme Gradient Boosting (XGBoost) algorithm to build our predictor. Results demonstrated that p6mA outperformed other existing predictors using different datasets. Conclusions: p6mA can predict the methylation status of DNA adenines, using only sequence files. It may be used as a tool to help the study of 6mA distribution pattern. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to dna nitrogen methyladenine algorimthm, dna n6-methyladenine, machine learning, xgboost, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2020, Li, Jianwei; Huang, Yan; Cui, Qinghua; Zhou, Yuan published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was m6Acorr: an online tool for the correction and comparison of m6A methylation profiles. And the article contained the following:

The anal. and comparison of RNA m6A methylation profiles have become increasingly important for understanding the post-transcriptional regulations of gene expression. However, current m6A profiles in public databases are not readily intercomparable, where heterogeneous profiles from the same exptl. report but different cell types showed unwanted high correlations. Several normalizing or correcting methods were tested to remove such laboratory bias. And m6Acorr, an effective pipeline for correcting m6A profiles, was presented on the basis of quantile normalization and empirical Bayes batch regression method. m6Acorr could efficiently correct laboratory bias in the simulated dataset and real m6A profiles in public databases. The preservation of biol. signals was examined after correction, and m6Acorr was found to better preserve differential methylation signals, m6A regulated targets, and m6A-related biol. features than alternative methods. Finally, the m6Acorr server was established. This server could eliminate the potential laboratory bias in m6A methylation profiles and perform profile-profile comparisons and functional anal. of hyper- (hypo-) methylated genes based on corrected methylation profiles. M6Acorr was established to correct the existing laboratory bias in RNA m6A methylation profiles and perform profile comparisons on the corrected datasets. The m6Acorr server is available at http://www.rnanut.net/m6Acorr. A stand-alone version with the correction function is also available in GitHub at https://github.com/emersON106/m6Acorr. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine

The Article related to m6acorr methylation profile mettl3 mettl14, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Liu, Chang; Li, Linshuang; Yang, Bo; Zhao, Yiqing; Dong, Xiyuan; Zhu, Lixia; Ren, Xinling; Huang, Bo; Yue, Jing; Jin, Lei; Zhang, Hanwang; Wang, Lan published an article.Application of 443-72-1 The title of the article was Transcriptome-wide N6-methyladenine methylation in granulosa cells of women with decreased ovarian reserve. And the article contained the following:

The emerging epitranscriptome plays an essential role in female fertility. As the most prevalent internal mRNA modification, N6-methyladenine (m6A) methylation regulate mRNA fate and translational efficiency. However, whether m6A methylation was involved in the aging-related ovarian reserve decline has not been investigated. Herein, we performed m6A transcriptome-wide profiling in the ovarian granulosa cells of younger women (younger group) and older women (older group). M6A methylation distribution was highly conserved and enriched in the CDS and 3’UTR region. Besides, an increased number of m6A methylated genes were identified in the older group. Bioinformatics anal. indicated that m6A methylated genes were enriched in the FoxO signaling pathway, adherens junction, and regulation of actin cytoskeleton. A total of 435 genes were differently expressed in the older group, moreover, 58 of them were modified by m6A. Several specific genes, including BUB1B, PHC2, TOP2A, DDR2, KLF13, and RYR2 which were differently expressed and modified by m6A, were validated using qRT-PCR and might be involved in the decreased ovarian functions in the aging ovary. Hence, our finding revealed the transcriptional significance of m6A modifications and provide potential therapeutic targets to promote fertility reservation for aging women. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to human ovarian reserve methyladenine methylation transcriptome granulosa cell, aging, granulosa cell, n6-methyladenosine, ovary, rna modification, Mammalian Pathological Biochemistry: Obstetrics – Gynecology and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem