Category: 443-72-1

Boulias, Konstantinos; Greer, Eric Lieberman published an article in 2021, the title of the article was Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS.Formula: C6H7N5 And the article contains the following content:

DNA methylation serves to mark DNA as either a directed epigenetic signaling modification or in response to DNA lesions. Methods for detecting DNA methylation have become increasingly more specific and sensitive over time. Conventional methods for detecting DNA methylation, ranging from paper chromatog. to differential restriction enzyme digestion preference to dot blots, have more recently been supplemented by ultrahigh performance liquid chromatog. coupled with mass spectrometry (UHPLC-MS/MS) to accurately quantify specific DNA methylation. Methylated DNA can also be sequenced by either methylated DNA immunoprecipitation followed by sequencing (MeDIP-seq) or single-mol. real-time sequencing (SMRTseq) for identifying genomic locations of DNA methylation. Here we describe a protocol for the detection and quantification of epigenetic signaling DNA methylation modifications including, N6-methyladenine (6mA), N4-methylcytosine (4mC) and C5-methylcytosine (5mC) in genomic DNA by triple quadrupole liquid chromatog. coupled with tandem mass spectrometry (QQQ-LC-MS/MS). The high sensitivity of the UHPLC-MS/MS methodol. and the use of calibration standards of pure nucleosides allow for the accurate quantification of DNA methylation. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to genomic dna methylation uhplc ms, 4mc, 5mc, 6ma, c5-methylcytosine, n4-methylcytosine, n6-methyladenine, uhplc-ms/ms, methylated dna, Biochemical Methods: Chromatographic and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On July 23, 2020, Li, Zheng; Zhao, Shuai; Nelakanti, Raman V.; Lin, Kaixuan; Wu, Tao P.; Alderman, Myles H. III; Guo, Cheng; Wang, Pengcheng; Zhang, Min; Min, Wang; Jiang, Zongliang; Wang, Yinsheng; Li, Haitao; Xiao, Andrew Z. published an article.COA of Formula: C6H7N5 The title of the article was N6-methyladenine in DNA antagonizes SATB1 in early development. And the article contained the following:

Abstract: The recent discovery of N6-methyladenine (N6-mA) in mammalian genomes suggests that it may serve as an epigenetic regulatory mechanism1. However, the biol. role of N6-mA and the mol. pathways that exert its function remain unclear. Here we show that N6-mA has a key role in changing the epigenetic landscape during cell fate transitions in early development. We found that N6-mA is upregulated during the development of mouse trophoblast stem cells, specifically at regions of stress-induced DNA double helix destabilization (SIDD)2-4. Regions of SIDD are conducive to topol. stress-induced unpairing of the double helix and have critical roles in organizing large-scale chromatin structures3,5,6. We show that the presence of N6-mA reduces the in vitro interactions by more than 500-fold between SIDD and SATB1, a crucial chromatin organizer that interacts with SIDD regions. Deposition of N6-mA also antagonizes SATB1 function in vivo by preventing its binding to chromatin. Concordantly, N6-mA functions at the boundaries between euchromatin and heterochromatin to restrict the spread of euchromatin. Repression of SIDD-SATB1 interactions mediated by N6-mA is essential for gene regulation during trophoblast development in cell culture models and in vivo. Overall, our findings demonstrate an unexpected mol. mechanism for N6-mA function via SATB1, and reveal connections between DNA modification, DNA secondary structures and large chromatin domains in early embryonic development. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).COA of Formula: C6H7N5

The Article related to methyladenine dna methylation satb1 embryogenesis mouse trophoblast, Biochemical Genetics: Genomic Processes and other aspects.COA of Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Alriquet, Marion; Calloni, Giulia; Martinez-Limon, Adrian; Ponti, Riccardo Delli; Hanspach, Gerd; Hengesbach, Martin; Tartaglia, Gian G.; Vabulas, R. Martin published an article in 2020, the title of the article was The protective role of m1A during stress-induced granulation.Quality Control of N-Methyl-7H-purin-6-amine And the article contains the following content:

Post-transcriptional methylation of N6-adenine and N1-adenine can affect transcriptome turnover and translation. Furthermore, the regulatory function of N6-methyladenine (m6A) during heat shock has been uncovered, including the enhancement of the phase separation potential of RNAs. In response to acute stress, e.g. heat shock, the orderly sequestration of mRNAs in stress granules (SGs) is considered important to protect transcripts from the irreversible aggregation. Until recently, the role of N1-methyladenine (m1A) on mRNAs during acute stress response remains largely unknown. Here we show that the methyltransferase complex TRMT6/61A, which generates the m1A tag, is involved in transcriptome protection during heat shock. Our bioinformatics anal. indicates that occurrence of the m1A motif is increased in mRNAs known to be enriched in SGs. Accordingly, the m1A-generating methyltransferase TRMT6/61A accumulated in SGs and mass spectrometry confirmed enrichment of m1A in the SG RNAs. The insertion of a single methylation motif in the untranslated region of a reporter RNA leads to more efficient recovery of protein synthesis from that transcript after the return to normal temperature Our results demonstrate far-reaching functional consequences of a minimal RNA modification on N1-adenine during acute proteostasis stress. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to transcriptome n1 methyladenine protective role granulation, n1-methyladenine, stress granules, stress response, Mammalian Pathological Biochemistry: Other and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2021, Khanal, Jhabindra; Lim, Dae Young; Tayara, Hilal; Chong, Kil To published an article.Related Products of 443-72-1 The title of the article was A stacking ensemble-based computational prediction of DNA N6-methyladenine (6mA) sites in the Rosaceae genome. And the article contained the following:

DNA N6-methyladenine (6 mA) is an epigenetic modification that plays a vital role in a variety of cellular processes in both eukaryotes and prokaryotes. Accurate information of 6 mA sites in the Rosaceae genome may assist in understanding genomic 6 mA distributions and various biol. functions such as epigenetic inheritance. Various studies have shown the possibility of identifying 6 mA sites through experiments, but the procedures are time-consuming and costly. To overcome the drawbacks of exptl. methods, we propose an accurate computational paradigm based on a machine learning (ML) technique to identify 6 mA sites in Rosa chinensis (R.chinensis) and Fragaria vesca (F.vesca). To improve the performance of the proposed model and to avoid overfitting, a recursive feature elimination with cross-validation (RFECV) strategy is used to extract the optimal number of features (ONF) subset from five different DNA sequence encoding schemes, i.e., Binary Encoding (BE), Ring-Function-Hydrogen-Chem. Properties (RFHC), Electron-Ion-Interaction Pseudo Potentials of Nucleotides (EIIP), Dinucleotide Physicochem. Properties (DPCP), and Trinucleotide Physicochem. Properties (TPCP). Subsequently, we use the ONF subset to train a double layers of ML-based stacking model to create a bioinformatics tool named ‘i6mA-stack’. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Related Products of 443-72-1

The Article related to rosaceae genome dna n6 methyladenine computational prediction, dna n6-methyladenine, machine learning, rfecv, sequence analysis, stacking, Biochemical Genetics: Genomic Processes and other aspects.Related Products of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 4, 2021, Zhu, Pengpeng; He, Fang; Hou, Yixuan; Tu, Gang; Li, Qiao; Jin, Ting; Zeng, Huan; Qin, Yilu; Wan, Xueying; Qiao, Yina; Qiu, Yuxiang; Teng, Yong; Liu, Manran published an article.Category: imidazoles-derivatives The title of the article was A novel hypoxic long noncoding RNA KB-1980E6.3 maintains breast cancer stem cell stemness via interacting with IGF2BP1 to facilitate c-Myc mRNA stability. And the article contained the following:

Abstract: The hostile hypoxic microenvironment takes primary responsibility for the rapid expansion of breast cancer tumors. However, the underlying mechanism is not fully understood. Here, using RNA sequencing (RNA-seq) anal., we identified a hypoxia-induced long noncoding RNA (lncRNA) KB-1980E6.3, which is aberrantly upregulated in clin. breast cancer tissues and closely correlated with poor prognosis of breast cancer patients. The enhanced lncRNA KB-1980E6.3 facilitates breast cancer stem cells (BCSCs) self-renewal and tumorigenesis under hypoxic microenvironment both in vitro and in vivo. Mechanistically, lncRNA KB-1980E6.3 recruited insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) to form a lncRNA KB-1980E6.3/IGF2BP1/c-Myc signaling axis that retained the stability of c-Myc mRNA through increasing binding of IGF2BP1 with m6A-modified c-Myc coding region instability determinant (CRD) mRNA. In conclusion, we confirm that lncRNA KB-1980E6.3 maintains the stemness of BCSCs through lncRNA KB-1980E6.3/IGF2BP1/c-Myc axis and suggest that disrupting this axis might provide a new therapeutic target for refractory hypoxic tumors. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Category: imidazoles-derivatives

The Article related to hypoxia lncrnkb1980e63 breast cancer stem cell igf2bp1 cmyc transcription, Biochemical Genetics: Genomic Processes and other aspects.Category: imidazoles-derivatives

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On July 30, 2020, Sun, Tong; Wu, Zhikun; Wang, Xiufang; Wang, Yilin; Hu, Xiaoyun; Qin, Wenyan; Lu, Senxu; Xu, Dongping; Wu, Yutong; Chen, Qiuchen; Ding, Xiangyu; Guo, Hao; Li, Yalun; Wang, Yuanhe; Fu, Boshi; Yao, Weifan; Wei, Minjie; Wu, Huizhe published an article.Quality Control of N-Methyl-7H-purin-6-amine The title of the article was LNC942 promoting METTL14-mediated m6A methylation in breast cancer cell proliferation and progression. And the article contained the following:

Abstract: Increasing evidence supports that long noncoding RNAs (lncRNAs) act as master regulators involved in tumorigenesis and development at the N6-methyladenine (m6A) epigenetic modification level. However, the underlying regulatory mechanism in breast cancer (BRCA) remains elusive. Here, we unveil that LINC00942 (LNC942) exerts its functions as an oncogene in promoting METTL14-mediated m6A methylation and regulating the expression and stability of its target genes CXCR4 and CYP1B1 in BRCA initiation and progression. Specifically, LNC942 and METTL14 were significantly upregulated accompanied with the upregulation of m6A levels in BRCA cells and our included BRCA cohorts (n = 150). Functionally, LNC942 elicits potent oncogenic effects on promoting cell proliferation and colony formation and inhibiting cell apoptosis, subsequently elevating METTL14-mediated m6A methylation levels and its associated mRNA stability and protein expression of CXCR4 and CYP1B1 in BRCA cells. Mechanistically, LNC942 directly recruits METTL14 protein by harboring the specific recognize sequence (+176-+265), thereby stabilized the expression of downstream targets of LNC942 including CXCR4 and CYP1B1 through posttranscriptional m6A methylation modification in vitro and in vivo. Therefore, our results uncover a novel LNC942-METTL14-CXCR4/CYP1B1 signaling axis, which provides new targets and crosstalk m6A epigenetic modification mechanism for BRCA prevention and treatment. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to lnc942 mettl14 methylation human breast cancer proliferation metastasis, Biochemical Genetics: Genomic Processes and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2021, Rahman, Chowdhury Rafeed; Amin, Ruhul; Shatabda, Swakkhar; Toaha, Sadrul Islam Md. published an article.Related Products of 443-72-1 The title of the article was A convolution based computational approach towards DNA N6-methyladenine site identification and motif extraction in rice genome. And the article contained the following:

DNA N6-methylation (6mA) in Adenine nucleotide is a post replication modification responsible for many biol. functions. Automated and accurate computational methods can help to identify 6mA sites in long genomes saving significant time and money. Our study develops a convolutional neural network (CNN) based tool i6mA-CNN capable of identifying 6mA sites in the rice genome. Our model coordinates among multiple types of features such as PseAAC (Pseudo Amino Acid Composition) inspired customized feature vector, multiple one hot representations and dinucleotide physicochem. properties. It achieves auROC (area under Receiver Operating Characteristic curve) score of 0.98 with an overall accuracy of 93.97% using fivefold cross validation on benchmark dataset. Finally, we evaluate our model on three other plant genome 6mA site identification test datasets. Results suggest that our proposed tool is able to generalize its ability of 6mA site identification on plant genomes irresp. of plant species. An algorithm for potential motif extraction and a feature importance anal. procedure are two by products of this research. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Related Products of 443-72-1

The Article related to dna nmethyladenine motif extraction rice genome computational method, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.Related Products of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On August 31, 2022, Shi, Hua; Li, Shuang; Su, Xi published an article.HPLC of Formula: 443-72-1 The title of the article was Plant6mA: A predictor for predicting N6-methyladenine sites with lightweight structure in plant genomes. And the article contained the following:

N6-methyladenine (6mA) in DNA, a type of DNA methylation in epigenetic modification, has attracted extensive attention in recent years. In order to improve our understanding of 6mA biol. activities and mechanisms in plant genomes, we need to be able to accurately identify 6mA sites. Because traditional wet-lab experiments frequently necessitate a large amount of manpower and time, a plethora of computational methods, particularly machine learning, have emerged to achieve fast and accurate 6mA site prediction. Traditional machine learning methods, on the other hand, rely heavily on manual features and integrated learning to improve performance, resulting in a reliance on prior knowledge and a large model scale. Furthermore, many models are only trained and tested for one species, with no comparison of model generalization performance, resulting in models with limited practical usability. In order to increase the generalization capability of the model, we propose a lightweight structure predictor Plant6mA based on Transformer encoder. Based on results on independent test sets, our proposed Plant6mA has better generalization performance than the most advanced methods in predicting 6mA location in plant genomes. Plant6mAs MultiHead Attention mechanism effectively enhances its expressive ability by capturing potential biol. information from multiple scales of the input sequence. Furthermore, we used a dimensionality reduction tool to visualize Plant6mAs training process and visually demonstrate the effectiveness of our model. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to plant6ma plant genome n6 methyladenine lightweight structure, bioinformatics, dna methylation, deep learning, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.HPLC of Formula: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Yuan, De-Hui; Xing, Jian-Feng; Luan, Mei-Wei; Ji, Kai-Kai; Guo, Jun; Xie, Shang-Qian; Zhang, Yuan-Ming published an article in 2020, the title of the article was DNA N6-methyladenine modification in wild and cultivated soybeans reveals different patterns in nucleus and cytoplasm.Computed Properties of 443-72-1 And the article contains the following content:

DNA 6mA modification, an important newly discovered epigenetic mark, plays a crucial role in organisms and has been attracting more and more attention in recent years. The soybean is economically the most important bean in the world, providing vegetable protein for millions of people. However, the distribution pattern and function of 6mA in soybean are still unknown. In this study, we decoded 6mA modification to single-nucleotide resolution in wild and cultivated soybeans, and compared the 6mA differences between cytoplasmic and nuclear genomes and between wild and cultivated soybeans. The motif of 6mA in the nuclear genome was conserved across the two kinds of soybeans, and ANHGA was the most dominant motif in wild and cultivated soybeans. Genes with 6mA modification in the nucleus had higher expression than those without modification. Interestingly, 6mA distribution patterns in cytoplasm for each soybean were significantly different from those in nucleus, which was reported for the first time in soybean. Our research provides a new insight in the deep anal. of cytoplasmic genomic DNA modification in plants. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Computed Properties of 443-72-1

The Article related to glycine genomic dna n6methyladenine nucleus cytoplasm, cultivated soybean, cytoplasm, methylation, nucleus, wild soybean, Plant Biochemistry: Classical Genetics and Phylogeny and other aspects.Computed Properties of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Douvlataniotis, Karolos; Bensberg, Maike; Lentini, Antonio; Gylemo, Bjoern; Nestor, Colm E. published an article in 2020, the title of the article was No evidence for DNA N6 -methyladenine in mammals.HPLC of Formula: 443-72-1 And the article contains the following content:

N6 -methyladenine (6mdA) is a widespread DNA modification in bacteria. More recently, 6mdA has also been characterized in mammalian DNA. However, measurements of 6mdA abundance and profiles are often very dissimilar between studies, even when performed on DNA from identical mammalian cell types. Using comprehensive bioinformatics analyses of published data and novel exptl. approaches, we reveal that efforts to assay 6mdA in mammals have been severely compromised by bacterial contamination, RNA contamination, technol. limitations, and antibody nonspecificity. These complications render 6mdA an exceptionally problematic DNA modification to study and have resulted in erroneous detection of 6mdA in several mammalian systems. Together, our results strongly imply that the evidence published to date is not sufficient to support the presence of 6mdA in mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to dna n methyladenine mammalian cell, Mammalian Biochemistry: Classical Genetics and Phylogeny and other aspects.HPLC of Formula: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem