Category: 443-72-1

Lowe, Paige; Olinski, Ryszard; Ruzov, Alexey published an article in 2021, the title of the article was Evidence for Noncytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview.Name: N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. Cytosine DNA methylation (5-methylcytsone, 5mC) is the major DNA modification found in the genomes of animals and plants. Although the roles of 5mC and its oxidized derivatives in the regulation of gene expression are relatively well attested and extensively explored, a number of recent studies imply that noncytosine DNA modifications may also convey specific biol. functions and act as “epigenetic” marks in multicellular organisms. Here we review exptl. evidence for the presence of noncytosine epigenetic modifications in metazoans and plants focusing on two “unusual” DNA bases, 5-hydroxymethyluracil (5hmU) and N6-methyladenine (6mA), and suggest potential explanations for inconsistencies in the currently available data on abundance and potential biol. roles of these DNA modifications in mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to review multicellular eukaryote noncytosine epigenetic dna modification, 5-hydroxymethyluracil, dna modifications, epigenetics, n6-methyladenine, Biochemical Genetics: Reviews and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Li, Xuwen; Guo, Shiyuan; Cui, Yan; Zhang, Zijian; Luo, Xinlong; Angelova, Margarita T.; Landweber, Laura F.; Wang, Yinsheng; Wu, Tao P. published an article.Synthetic Route of 443-72-1 The title of the article was NT-seq: a chemical-based sequencing method for genomic methylome profiling. And the article contained the following:

Abstract: DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N6-methyladenine (6mA), N4-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to escherichia helicobacter nt seq genome methylome dna methylation, dna methylation, next-generation sequencing, whole-genome epigenetic profiling, Biochemical Genetics: Methods and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Bochtler, Matthias; Fernandes, Humberto published an article in 2021, the title of the article was DNA adenine methylation in eukaryotes: Enzymatic mark or a form of DNA damage.SDS of cas: 443-72-1 And the article contains the following content:

A Review. 6-Methyladenine (6mA) is fairly abundant in nuclear DNA of basal fungi, ciliates and green algae. In these organisms, 6mA is maintained near transcription start sites in ApT context by a parental-strand instruction dependent maintenance methyltransferase and is pos. associated with transcription. In animals and plants, 6mA levels are high only in organellar DNA. The 6mA levels in nuclear DNA are very low. They are attributable to nucleotide salvage and the activity of otherwise mitochondrial METTL4, and may be considered as a price that cells pay for adenine methylation in RNA and/or organellar DNA. Cells minimize this price by sanitizing dNTP pools to limit 6mA incorporation, and by converting 6mA that has been incorporated into DNA back to adenine. Hence, 6mA in nuclear DNA should be described as an epigenetic mark only in basal fungi, ciliates and green algae, but not in animals and plants. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to review dna adenine methylation eukaryotes, 6ma, dna damage, dna modifications, cancer, epitranscriptome/epigenome, nucleotide salvage, transcription, Biochemical Genetics: Reviews and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 1, 2020, Wu, Kou-Juey published an article.Computed Properties of 443-72-1 The title of the article was The epigenetic roles of DNA N6-Methyladenine (6mA) modification in eukaryotes. And the article contained the following:

A review. The DNA N6-methyladenine (6mA) modification is a prevalent epigenetic mark in prokaryotes, but the low abundance of 6mA in eukaryotes has recently received attention. The possible role of 6mA as an epigenetic mark in eukaryotes is starting to be recognized. This review article addresses the epigenetic roles of 6mA in eukaryotes. The existence of 6mA in metazoans and plants, the correlation of 6mA with gene expression, the enzymes catalyzing the deposition and removal of the 6mA modification, the relationship of 6mA to nucleosome positioning, the 6mA interaction with chromatin, its role in tumorigenesis and other physiol. conditions/diseases and tech. issues in 6mA detection/profiling and bioinformatics anal. are described. New directions and unresolved issues (e.g., the base-pair-resolution 6mA-sequencing method and gene activation vs. repression) in 6mA research are discussed. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Computed Properties of 443-72-1

The Article related to review epigenetic mark n6methyladenine tumorigenesis, chromatin, demethylase, epigenetic mark, gene expression, methyltransferase, nucleosome positioning, Biochemical Genetics: Reviews and other aspects.Computed Properties of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On May 31, 2020, Hasan, Mehedi Md.; Manavalan, Balachandran; Shoombuatong, Watshara; Khatun, Mst. Shamima; Kurata, Hiroyuki published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was i6mA-Fuse: improved and robust prediction of DNA 6 mA sites in the Rosaceae genome by fusing multiple feature representation. And the article contained the following:

DNA N6-methyladenine (6 mA) is one of the most vital epigenetic modifications and involved in controlling the various gene expression levels. With the avalanche of DNA sequences generated in numerous databases, the accurate identification of 6 mA plays an essential role for understanding mol. mechanisms. Because the exptl. approaches are time-consuming and costly, it is desirable to develop a computation model for rapidly and accurately identifying 6 mA. To the best of our knowledge, we first proposed a computational model named i6mA-Fuse to predict 6 mA sites from the Rosaceae genomes, especially in Rosa chinensis and Fragaria vesca. We implemented the five encoding schemes, i.e., mononucleotide binary, dinucleotide binary, k-space spectral nucleotide, k-mer, and electron-ion interaction pseudo potential compositions, to build the five, single-encoding random forest (RF) models. The i6mA-Fuse uses a linear regression model to combine the predicted probability scores of the five, single encoding-based RF models. The resultant species-specific i6mA-Fuse achieved remarkably high performances with AUCs of 0.982 and 0.978 and with MCCs of 0.869 and 0.858 on the independent datasets of Rosa chinensis and Fragaria vesca, resp. In the F. vesca-specific i6mA-Fuse, the MBE and EIIP contributed to 75% and 25% of the total prediction; in the R. chinensis-specific i6mA-Fuse, Kmer, MBE, and EIIP contribute to 15%, 65%, and 20% of the total prediction. To assist high-throughput prediction for DNA 6 mA identification, the i6mA-Fuse is publicly accessible at https://kurata14.bio.kyutech.ac.jp/i6mA-Fuse/. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to methyladenine fuse dna genome rosa fragaria, dna 6 ma, feature encoding, machine learning, sequence analysis, Biochemical Methods: Apparatus and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2022, Hui, Wenyan; Zhang, Wenyi; Li, Jing; Kwok, Lai-Yu; Zhang, Heping; Kong, Jian; Sun, Tiansong published an article.HPLC of Formula: 443-72-1 The title of the article was Functional analysis of the second methyltransferase in the bacteriophage exclusion system of Lactobacillus casei Zhang. And the article contained the following:

The antiphage ability is an important feature of fermentation strains in the dairy industry. Our previous work described the bacteriophage exclusion (BREX) system in the probiotic strain, Lactobacillus casei Zhang. The function of L. casei Zhang pglX gene in mediating 5′-ACRCm6AG-3′ methylation was also confirmed. This study aimed to further dissect the function of the BREX system of L. casei Zhang by inactivating its second methyltransferase gene (LCAZH_2054). The methylome of the mutant, L. casei Zhang Δ2054, was profiled by single-mol. real-time sequencing. Then, the cell morphol., growth, plasmid transformation efficiency, and stability of the wildtype and mutant were compared. The mutant did not have an observable effect in microscopic and colony morphol., but it reached a higher cell d. after entering the exponential phase without obvious increase in the cell viability. The mutant had fewer 5′-ACRCm6AG-3′ methylation compared with the wildtype (1835 vs. 1906). Interestingly, no significant difference was observed in the transformation efficiency between the 2 strains when plasmids without cognate recognition sequence (pSec:Leiss:Nuc and pG+host9) were transformed, contrasting to transforming cells with cognate recognition sequence-containing plasmids (pMSP3535 and pTRKH2). The efficiency of transforming pMSP3535 into the LCAZH_2054 mutant was significantly lower than the wildtype, whereas an opposite trend was seen in pTRKH2 transformation. Moreover, compared with the wildtype, the mutant strain had higher capacity in retaining pMSP3535 and lower capacity in retaining pTRKH2, suggesting an unequal tolerance level to different foreign DNA. In conclusion, LCAZH_2054 was not directly responsible for 5′-ACRCm6AG-3′ methylation in L. casei Zhang, but it might help regulate the function and specificity of the BREX system. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1

The Article related to methyltransferase bacteriophage lactobacillus, lactobacillus casei zhang, bacteriophage exclusion system, methyltransferase, phage infection, transformation efficiency, Food and Feed Chemistry: Other and other aspects.HPLC of Formula: 443-72-1

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Fernandes, Sara B.; Grova, Nathalie; Roth, Sarah; Duca, Radu Corneliu; Godderis, Lode; Guebels, Pauline; Meriaux, Sophie B.; Lumley, Andrew I.; Bouillaud-Kremarik, Pascaline; Ernens, Isabelle; Devaux, Yvan; Schroeder, Henri; Turner, Jonathan D. published an article in 2021, the title of the article was N6-methyladenine in eukaryotic DNA: tissue distribution, early embryo development, and neuronal toxicity.Product Details of 443-72-1 And the article contains the following content:

DNA methylation is one of the most important epigenetic modifications and is closely related with several biol. processes such as regulation of gene transcription and the development of non-malignant diseases. The prevailing dogma states that DNA methylation in eukaryotes occurs essentially through 5-methylcytosine (5mC) but recently adenine methylation was also found to be present in eukaryotes. In mouse embryonic stem cells, 6-methyladenine (6mA) was associated with the repression and silencing of genes, particularly in the X-chromosome, known to play an important role in cell fate determination Here, we have demonstrated that 6mA is a ubiquitous eukaryotic epigenetic modification that is put in place during epigenetically sensitive periods such as embryogenesis and fetal development. In somatic cells there are clear tissue specificity in 6mA levels, with the highest 6mA levels being observed in the brain. In zebrafish, during the first 120 h of embryo development, from a single pluripotent cell to an almost fully formed individual, 6mA levels steadily increase. An identical pattern was observed over embryonic days 7-21 in the mouse. Furthermore, exposure to a neurotoxic environmental pollutant during the same early life period may led to a decrease in the levels of this modification in female rats. The identification of the periods during which 6mA epigenetic marks are put in place increases our understanding of this mammalian epigenetic modification, and raises the possibility that it may be associated with developmental processes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to methyladenine eukaryotic dna early embryo development neuronal toxicity, 6-methyladenine, dna methylation, brain, developmental neurotoxicity, embryo development, stress, Pharmaceuticals: Pharmaceutics and other aspects.Product Details of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Li, Hao; Zhang, Ning; Wang, Yuechen; Xia, Siyuan; Zhu, Yating; Xing, Chen; Tian, Xuefeng; Du, Yinan published an article in 2022, the title of the article was DNA N6-methyladenine modification in eukaryotic genome.Name: N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. DNA methylation is treated as an important epigenetic mark in various biol. activities. In the past, a large number of articles focused on 5 mC while lacking attention to N6-methyladenine (6 mA). The presence of 6 mA modification was previously discovered only in prokaryotes. Recently, with the development of detection technologies, 6 mA has been found in several eukaryotes, including protozoans, metazoans, plants, and fungi. The importance of 6 mA in prokaryotes and single-celled eukaryotes has been widely accepted. However, due to the incredibly low d. of 6 mA and restrictions on detection technologies, the prevalence of 6 mA and its role in biol. processes in eukaryotic organisms are highly debated. In this review, we first summarize the advantages and disadvantages of 6 mA detection methods. Then, we conclude existing reports on the prevalence of 6 mA in eukaryotic organisms. Next, we highlight possible methyltransferases, demethylases, and the recognition proteins of 6 mA. In addition, we summarize the functions of 6 mA in eukaryotes. Last but not least, we summarize our point of view and put forward the problems that need further research. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to review dna methyladenine modification eukaryotic genome, dna modification, n6-methyladenine, epigenetics, eukaryotic genome, methylation, Mammalian Biochemistry: Reviews and other aspects.Name: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Zhang, Ying; Liu, Yan; Xu, Jian; Wang, Xiaoyu; Peng, Xinxin; Song, Jiangning; Yu, Dong-Jun published an article in 2021, the title of the article was Leveraging the attention mechanism to improve the identification of DNA N6-methyladenine sites.Formula: C6H7N5 And the article contains the following content:

DNA N6-methyladenine is an important type of DNA modification that plays important roles in multiple biol. processes. Despite the recent progress in developing DNA 6mA site prediction methods, several challenges remain to be addressed. For example, although the hand-crafted features are interpretable, they contain redundant information that may bias the model training and have a neg. impact on the trained model. Furthermore, although deep learning (DL)-based models can perform feature extraction and classification automatically, they lack the interpretability of the crucial features learned by those models. As such, considerable research efforts have been focused on achieving the trade-off between the interpretability and straight forwardness of DL neural networks. In this study, we develop two new DL-based models for improving the prediction of N6-methyladenine sites, termed LA6mA and AL6mA, which use bidirectional long short-term memory to resp. capture the long-range information and self-attention mechanism to extract the key position information from DNA sequences. The performance of the two proposed methods is benchmarked and evaluated on the two model organisms Arabidopsis thaliana and Drosophila melanogaster. On the two benchmark datasets, LA6mA achieves an area under the receiver operating characteristic curve (AUROC) value of 0.962 and 0.966, whereas AL6mA achieves an AUROC value of 0.945 and 0.941, resp. Moreover, an in-depth anal. of the attention matrix is conducted to interpret the important information, which is hidden in the sequence and relevant for 6mA site prediction. The two novel pipelines developed for DNA 6mA site prediction in this work will facilitate a better understanding of the underlying principle of DL-based DNA methylation site prediction and its future applications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5

The Article related to arabidopsis drosophila dna n6 methyladenine, 6ma, dna modification, lstm, attention interpretation, deep learning, self-attention mechanism, Biochemical Methods: Biological and other aspects.Formula: C6H7N5

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Wan, Liqi; Lam, Sik Lok; Lee, Hung Kay; Guo, Pei published an article in 2021, the title of the article was Effects of adenine methylation on the structure and thermodynamic stability of a DNA minidumbbell.Recommanded Product: 443-72-1 And the article contains the following content:

DNA methylation is a prevalent regulatory modification in prokaryotes and eukaryotes. N1-methyladenine (m1A) and N6-methyladenine (m6A) have been found to be capable of altering DNA structures via disturbing Watson-Crick base pairing. However, little has been known about their influences on non-B DNA structures, which are associated with genetic instabilities. In this work, we investigated the effects of m1A and m6A on both the structure and thermodn. stability of a newly reported DNA minidumbbell formed by two TTTA tetranucleotide repeats. As revealed by the results of NMR spectroscopic studies, both m1A and m6A favored the formation of a T·m1A and T·m6A Hoogsteen base pair, resp. More intriguingly, the m1A and m6A modifications brought about stabilization and destabilization effects on the DNA minidumbbell, resp. This work provides new biophys. insights into the effects of adenine methylation on the structure and thermodn. stability of DNA. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: 443-72-1

The Article related to adenine methylation dna minidumbbell thermodn stability, dna methylation, dna minidumbbell, n1-methyladenine, n6-methyladenine, nuclear magnetic resonance spectroscopy, Biochemical Methods: Biological and other aspects.Recommanded Product: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem