Category: 443-72-1

On September 30, 2020, Zhong, Hui; Tang, Hui-Fang; Kai, Yin published an article.SDS of cas: 443-72-1 The title of the article was N6-methyladenine RNA Modification (m6A): An Emerging Regulator of Metabolic Diseases. And the article contained the following:

A review. N6-methyladenine RNA modification (m6A) is an RNA methylation modification catalyzed by methyltransferase at the 6th position nitrogen atom of adenine (A), which is the most common chem. modification of eukaryotic mRNA (mRNA). Recently, m6A has been found to play an important role in the dynamic regulation of RNA, which is crucial for some physiol. and pathophysiol. processes such as adipogenesis, cell differentiation, and the immune/inflammatory response. Metabolic diseases are a series of chronic inflammatory disorders caused by metabolic dysfunction of proteins, glucose, and lipids. Emerging studies have shown that m6A plays an important role in the process of metabolic diseases such as obesity, type 2 diabetes mellitus (T2DM) and cardiovascular diseases (CVDs) via regulation of glucose/lipid metabolism and the immune/inflammatory response. In this review, we will summarize the role of m6A in metabolic diseases, which may provide new ideas for the prevention and treatment of metabolic diseases. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1

The Article related to review methyladenine rna modification metabolic disease, n6-adenine methylation, cardiovascular diseases, metabolic diseases, obesity, type 2 diabetes mellitus, Mammalian Pathological Biochemistry: Reviews and other aspects.SDS of cas: 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 30, 2021, Hu, Yibo; Zhao, Xiaohui published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was Role of m6A in osteoporosis, arthritis and osteosarcoma (Review). And the article contained the following:

A review. RNA modification is a type of post-transcriptional modification that regulates important cellular pathways, such as the processing and metabolism of RNA. The most abundant form of methylation modification is RNA N6-methyladenine (m6A), which plays various post-transcriptional regulatory roles in cellular biol. functions, including cell differentiation, embryonic development and disease occurrence. Bones play a pivotal role in the skeletal system as they support and protect muscles and other organs, facilitate movement and ensure haematopoiesis. The development and remodelling of bones require a delicate and accurate regulation of gene expression by epigenetic mechanisms that involve modifications of histone, DNA and RNA. The present review discusses the enzymes and proteins involved in mRNA m6A methylation modification and summarises current research progress and the mechanisms of mRNA m6A methylation in common orthopaedic diseases, including osteoporosis, arthritis and osteosarcoma. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to review osteoporosis arthritis osteosarcoma m6a, n6-methyladenine, common orthopaedic diseases, demethylase, methyltransferase, reader proteins, Mammalian Pathological Biochemistry: Reviews and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Bera, Amit; Lewis, Stephen M. published an article in 2020, the title of the article was Regulation of epithelial-to-mesenchymal transition by alternative translation initiation mechanisms and its implications for cancer metastasis.Safety of N-Methyl-7H-purin-6-amine And the article contains the following content:

A review. Translation initiation plays a critical role in the regulation of gene expression for development and disease conditions. During the processes of development and disease, cells select specific mRNAs to be translated by controlling the use of diverse translation initiation mechanisms. Cells often switch translation initiation from a cap-dependent to a cap-independent mechanism during epithelial-to-mesenchymal transition (EMT), a process that plays an important role in both development and disease. EMT is involved in tumor metastasis because it leads to cancer cell migration and invasion, and is also associated with chemoresistance. In this review we will provide an overview of both the internal ribosome entry site (IRES)-dependent and N6-methyladenosine (m6A)-mediated translation initiation mechanisms and discuss how cap-independent translation enables cells from primary epithelial tumors to achieve a motile mesenchymal-like phenotype, which in turn drives tumor metastasis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to review epithelial mesenchymal cancer metastasis, ires, itaf, cancer, epithelial-to-mesenchymal transition (emt), m6a-mediated translation, metastasis, Mammalian Pathological Biochemistry: Reviews and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On September 17, 2021, Mathur, Lavina; Jung, Sunhee; Jang, Cholsoon; Lee, Gina published an article.Safety of N-Methyl-7H-purin-6-amine The title of the article was Quantitative analysis of m6A RNA modification by LC-MS. And the article contained the following:

N6-adenosine methylation (m6A) of mRNA (mRNA) plays key regulatory roles in gene expression. Accurate measurement of m6A levels is thus critical to understand its dynamic changes in various biol. settings. Here, we provide a protocol to quantitate the levels of adenosine and m6A in cellular mRNAs. Using nuclease and phosphatase, we digest mRNA into nucleosides, which are subsequently quantified using liquid chromatog. mass spectrometry. For complete details on the use and execution of this protocol, please refer to Cho et al. (2021). The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to adenine: analogs & derivatives, adenine: analysis, adenine: chemistry, adenosine: analogs & derivatives, adenosine: chemistry, adenosine: metabolism, biochemical phenomena, chromatography, liquid: methods, methylation, nucleosides: analysis, rna: chemistry, rna, messenger: chemistry, rna and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Chen, Luyang; Zhang, Meiying; Guo, Mingzhou published an article in 2020, the title of the article was DNA N6-methyladenine increased in human esophageal squamous cell carcinoma..Product Details of 443-72-1 And the article contains the following content:

Esophageal cancer is one of the most common malignancies worldwide. DNA N6-methyladenine (6mA) has been well-studied in prokaryotes, while the distribution and biological functions of DNA 6mA in eukaryotic cells remain to be elucidated. Recently, DNA 6mA epigenetic modification was found in human gastric and liver cancers. To explore the status of DNA 6mA epigenetic modification in esophageal cancer, 101 cases of human esophageal squamous cell carcinoma (ESCC) and matched adjacent normal tissue samples were analyzed by dot blot assay. The levels of genomic DNA 6mA were significantly higher in ESCC tissue samples than in matched adjacent normal tissue samples (P<0.001). Increased DNA 6mA was associated with poor tumor differentiation (P<0.05), while no association was found between 6mA modification and gender, age, tumor size, TNM stage, lymph node metastasis, smoking, alcohol intake, or family history (all P>0.05). In conclusion, DNA 6mA epigenetic modification increased in human ESCC and may serve as a prognostic marker. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Product Details of 443-72-1

The Article related to adenine: analogs & derivatives, adenine: analysis, adenine: metabolism, adult, aged, aged, 80 and over, biomarkers, tumor: analysis, biomarkers, tumor: metabolism, dna methylation, epigenesis, genetic, esophageal mucosa: pathology, esophageal neoplasms: diagnosis, esophageal neoplasms: genetics and other aspects.Product Details of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Guo, Ye; Pei, Yuqing; Li, Kexin; Cui, Wei; Zhang, Donghong published an article in 2020, the title of the article was DNA N6-methyladenine modification in hypertension.Synthetic Route of 443-72-1 And the article contains the following content:

DNA methylation has a role in the pathogenesis of essential hypertension. DNA N6-methyladenine (6mA) modification as a novel adenine methylation exists in human tissues, but whether it plays a role in hypertension development remains unclear. Here, we reported that the global 6mA DNA level in leukocytes was significantly reduced in patients with hypertension and was reversed with successful treatment. Age, systolic blood pressure, and serum total cholesterol and high-d. lipoprotein levels were associated with decreased leukocyte 6mA DNA level. Elevated ALKBH1 (AlkB homolog 1), a demethylase of 6mA, level mediated this dynamic change in 6mA level in leukocytes and vascular smooth muscle cells in hypertension mouse and rat models. Knockdown of ALKBH1 suppressed angiotensin II-induced vascular smooth muscle phenotype transformation, proliferation and migration. ALKBH1-6mA directly and neg. regulated hypoxia inducible factor 1 α (HIF1α), which responded to angiotensin II-induced vascular remodeling. Collectively, our results demonstrate a potential epigenetic role for ALKBH1-6mA regulation in hypertension development, diagnosis and treatment. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to adenine: analogs & derivatives, adenine: metabolism, alkb homolog 1, histone h2a dioxygenase: genetics, angiotensin ii: metabolism, animals, dna methylation, dna repair enzymes, epigenesis, genetic, hypertension: blood, hypertension: metabolism, hypertension: therapy, leukocytes: metabolism, mice, muscle, smooth and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Shan, Li; Lu, Ye; Song, Yihua; Zhu, Xiaoli; Xiang, Cheng-Cheng; Zuo, Er-Dong; Cheng, Xu published an article in 2022, the title of the article was Identification of nine M6A-related long noncoding RNAs as prognostic signatures associated with oxidative stress in oral cancer based on data from the cancer genome atlas.Synthetic Route of 443-72-1 And the article contains the following content:

Objective. Although the expression of long noncoding RNAs (lncRNAs) and N6-methyladenosine (M6A) is correlated with different tumors, it remains unclear how M6A-related lncRNA functioning affects the initiation and progression of oral squamous cell carcinoma (OSCC). Materials and Methods. Gene expression and clin. data were retrieved from The Cancer Genome Atlas. The prognostic value of M6A-related lncRNAs was determined using univariate Cox regression analyses. Gene set enrichment anal. of OSCC patient clusters revealed the pathways that elucidate the mechanism. Furthermore, a risk-based model was established. The difference in the overall survival (OS) between groups, including low-/high-risk groups, was determined by Kaplan-Meier anal. Relationships among immune cells, clusters, clinicopathol. characteristics, and risk scores were explored. Results. Among 1,080 M6A-related lncRNAs, 36 were prognosis-related. Patients with OSCC were divided into two clusters. T stage and the pathol. grade were noticeably lower in cluster 2 than in cluster 1. Epithelial-mesenchymal transition showed greater enrichment in cluster 1. Nine hub M6A-related lncRNAs were identified for the model of risk score for predicting OSCC prognosis. The OS was longer in patients with a low-risk score than in patients with a high-risk score. The risk score was an independent prognostic factor of OSCC and was associated with the infiltration of different immune cells. T stages and the American Joint Committee on Cancer (AJCC) stages were more advanced in the high-risk score group than in the low-risk score group. Finally, expression correlation anal. showed that the risk score is associated with the expression of oxidative stress markers. Conclusion. M6A-related lncRNAs play an important role in OSCC progression. Immune cell infiltration was related to the risk score model in OSCC and could accurately predict OSCC prognosis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to adenine: analogs & derivatives, biomarkers, tumor: genetics, biomarkers, tumor: metabolism, carcinoma, squamous cell: diagnosis, carcinoma, squamous cell: genetics, carcinoma, squamous cell: pathology, gene expression regulation, neoplastic, head and neck neoplasms: diagnosis, head and neck neoplasms: genetics, humans and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On January 31, 2020, Zhang, Shuming; Li, Bianbian; Du, Ke; Liang, Tingting; Dai, Mengyuan; Huang, Wenxin; Zhang, Huizhi; Ling, Yihui; Zhang, Huidong published an article.Application of 443-72-1 The title of the article was Epigenetically modified N6-methyladenine inhibits DNA replication by human DNA polymerase iota. And the article contained the following:

Among human four Y-family DNA polymerases, hPol ι is exceptionally error-prone in DNA synthesis. 6 mA plays significant roles in epigenetic regulation of numerous biol. processes. Nonetheless, its effects on DNA replication by hPol ι is still unclear. In this work, we found that 6 mA and Hyp, the intermediate of 6 mA, inhibited the replication of DNA by hPol ι. 6 mA lost priority in extension beyond 6 mA:T pair, partially reducing dTTP incorporation efficiency and inhibiting next-base extension. Hyp was prone to dCTP incorporation and extension beyond Hyp:C instead of Hyp:T pair. Statistically, 6 mA primarily reduced the burst incorporation rate (kpol) and slightly increased the dissociation constant (Kd,dTTP). However, Hyp mainly increased the Kd,dCTP yet did not affect the kpol, both reducing the burst incorporation efficiency (kpol/Kd,dCTP). 6 mA together with Hyp weakened the binding affinity of hPol ι to DNA in binary or ternary complex. The misincorporation opposite 6 mA or Hyp further weakened this binding affinity. The Me group in 6 mA doesn′t almost affect the H-bond formation with dTTP, therefore mildly inhibiting dTTP incorporation. As an analog of G, Hyp can form only two H-bonds with dCTP, thus reducing dCTP incorporation. This work provides a new insight in how the epigenetically modified 6 mA and its intermediate Hyp affect replication of DNA by human DNA polymerase ι. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1

The Article related to dna polymerase, dna replication, human dna polymerase ι, hypoxanthine (hyp), n(6)-methyladenine (6 ma), steady-state and pre-steady-state kinetics, and other aspects.Application of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On March 31, 2022, Lin, Qu; Chen, Jun-wei; Yin, Hao; Li, Ming-an; Zhou, Chu-ren; Hao, Tao-fang; Pan, Tao; Wu, Chun; Li, Zheng-ran; Zhu, Duo; Wang, Hao-fan; Huang, Ming-sheng published an article.Synthetic Route of 443-72-1 The title of the article was DNA N6-methyladenine involvement and regulation of hepatocellular carcinoma development. And the article contained the following:

DNA N6-methyladenine (6 mA) is a new type of DNA methylation identified in various eukaryotic cells. However, its alteration and genomic distribution features in hepatocellular carcinoma (HCC) remain elusive. In this study, we found that N6AMT1 overexpression increased HCC cell viability, suppressed apoptosis, and enhanced migration and invasion, whereas ALKBH1 overexpression induced the opposite effects. Further, 23,779 gain-of-6 mA regions and 11,240 loss-of-6 mA regions were differentially identified in HCC tissues. The differential gain and loss of 6 mA regions were considerably enriched in intergenic regions. Moreover, 7% of the differential 6 mA modifications were associated with tumors, with 60 associated with oncogenes and 57 with tumor suppressor genes (TSGs), and 17 were common to oncogenes and TSGs. The candidate genes affected by 6 mA were filtered by gene ontol. (GO) and RNA-seq. Using quant. polymerase chain reaction (qPCR), BCL2 and PARTICL were found to be correlated with DNA 6 mA in certain HCC processes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1

The Article related to dna n6methyladenine regulation hepatocellular carcinoma development, alkbh1, genomic distribution, hepatocellular carcinoma, n6-methyladenine, n6amt1, Biochemical Genetics: Other and other aspects.Synthetic Route of 443-72-1

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On October 31, 2021, Li, Xuwen; Zhang, Zijian; Luo, Xinlong; Schrier, Jacob; Yang, Andrew D.; Wu, Tao P. published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was The exploration of N6-deoxyadenosine methylation in mammalian genomes. And the article contained the following:

A review. N6-methyladenine (N6-mA, m6dA, or 6mA), a prevalent DNA modification in prokaryotes, has recently been identified in higher eukaryotes, including mammals. Although 6mA has been well-studied in prokaryotes, the function and regulatory mechanism of 6mA in eukaryotes are still poorly understood. Recent studies indicate that 6mA can serve as an epigenetic mark and play critical roles in various biol. processes, from transposable-element suppression to environmental stress response. Here, we review the significant advances in methodol. for 6mA detection and major progress in understanding the regulation and function of this non-canonical DNA methylation in eukaryotes, predominantly mammals. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to review dna methylation mammalian genome, dna n6-methyladenine (6ma), mammalian dna modification, non-canonical mammalian dna methylation, Biochemical Genetics: Reviews and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem