Category: 443-72-1

Jenjaroenpun, Piroon; Wongsurawat, Thidathip; Wadley, Taylor D.; Wassenaar, Trudy M.; Liu, Jun; Dai, Qing; Wanchai, Visanu; Akel, Nisreen S.; Jamshidi-Parsian, Azemat; Franco, Aime T.; Boysen, Gunnar; Jennings, Michael L.; Ussery, David W.; He, Chuan; Nookaew, Intawat published an article in 2021, the title of the article was Decoding the epitranscriptional landscape from native RNA sequences.COA of Formula: C6H7N5 And the article contains the following content:

Traditional epitranscriptomics relies on capturing a single RNA modification by antibody or chem. treatment, combined with short-read sequencing to identify its transcriptomic location. This approach is labor-intensive and may introduce exptl. artifacts. Direct sequencing of native RNA using Oxford Nanopore Technologies (ONT) can allow for directly detecting the RNA base modifications, although these modifications might appear as sequencing errors. The percent Error of Specific Bases (%ESB) was higher for native RNA than unmodified RNA, which enabled the detection of ribonucleotide modification sites. Based on the %ESB differences, we developed a bioinformatic tool, epitranscriptional landscape inferring from glitches of ONT signals (ELIGOS), that is based on various types of synthetic modified RNA and applied to rRNA and mRNA. ELIGOS is able to accurately predict known classes of RNA methylation sites (AUC > 0.93) in rRNAs from Escherichia coli, yeast, and human cells, using either unmodified in vitro transcription RNA or a background error model, which mimics the systematic error of direct RNA sequencing as the reference The well-known DRACH/RRACH motif was localized and identified, consistent with previous studies, using differential anal. of ELIGOS to study the impact of RNA m6A methyltransferase by comparing wild type and knockouts in yeast and mouse cells. Lastly, the DRACH motif could also be identified in the mRNA of three human cell lines. The mRNA modification identified by ELIGOS is at the level of individual base resolution In summary, we have developed a bioinformatic software package to uncover native RNA modifications. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).COA of Formula: C6H7N5

The Article related to decoding epitranscriptional rna sequence, Placeholder for records without volume info and other aspects.COA of Formula: C6H7N5

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Imidazole | C3H4N2 – PubChem

Woodcock, Clayton B.; Horton, John R.; Zhou, Jujun; Bedford, Mark T.; Blumenthal, Robert M.; Zhang, Xing; Cheng, Xiaodong published an article in 2020, the title of the article was Biochemical and structural basis for YTH domain of human YTHDC1 binding to methylated adenine in DNA.Name: N-Methyl-7H-purin-6-amine And the article contains the following content:

The recently characterized mammalian writer (methyltransferase) and eraser (demethylase) of the DNA N6-methyladenine (N6mA) Me mark act on single-stranded (ss) and transiently-unpaired DNA. As YTH domain-containing proteins bind N6mA-containing RNA in mammalian cells, we investigated whether mammalian YTH domains are also Me mark readers of N6mA DNA. Here, we show that the YTH domain of YTHDC1 (known to localize in the nucleus) binds ssDNA containing N6mA, with a 10 nM dissociation constant This binding is stronger by a factor of 5 than in an RNA context, tested under the same conditions. However, the YTH domains of YTHDF2 and YTHDF1 (predominantly cytoplasmic) exhibited the opposite effect with �.5-2x stronger binding to ssRNA containing N6mA than to the corresponding DNA. We determined two structures of the YTH domain of YTHDC1 in complex with N6mA-containing ssDNA, which illustrated that YTHDC1 binds the methylated adenine in a single-stranded region flanked by duplexed DNA. We discuss the hypothesis that the writer-reader-eraser of N6mA-containining ssDNA is associated with maintaining genome stability. Structural comparison of YTH and SRA domains (the latter a DNA 5-methylcytosine reader) revealed them to be diverse members of a larger family of DNA/RNA modification readers, apparently having originated from bacterial modification-dependent restriction enzymes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to ythdc1 dna methylated adenine biochem structure, Placeholder for records without volume info and other aspects.Name: N-Methyl-7H-purin-6-amine

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Zhang, Guoqiang; Xu, Yongru; Wang, Xiaona; Zhu, Yuanxiang; Wang, Liangliang; Zhang, Wenxin; Wang, Yiru; Gao, Yajie; Wu, Xuna; Cheng, Ying; Sun, Qinmiao; Chen, Dahua published an article.Application In Synthesis of N-Methyl-7H-purin-6-amine The title of the article was Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay. And the article contained the following:

Maternal RNA degradation is critical for embryogenesis and is tightly controlled by maternal RNA-binding proteins. Fragile X mental-retardation protein (FMR1) binds target mRNAs to form ribonucleoprotein (RNP) complexes/granules that control various biol. processes, including early embryogenesis. However, how FMR1 recognizes target mRNAs and how FMR1-RNP granule assembly/disassembly regulates FMR1-associated mRNAs remain elusive. Here we show that Drosophila FMR1 preferentially binds mRNAs containing m6A-marked “AGACU” motif with high affinity to contributes to maternal RNA degradation The high-affinity binding largely depends on a hydrophobic network within FMR1 KH2 domain. Importantly, this binding greatly induces FMR1 granule condensation to efficiently recruit unmodified mRNAs. The degradation of maternal mRNAs then causes granule de-condensation, allowing normal embryogenesis. Our findings reveal that sequence-specific mRNAs instruct FMR1-RNP granules to undergo a dynamic phase-switch, thus contributes to maternal mRNA decay. This mechanism may represent a general principle that regulated RNP-granules control RNA processing and normal development. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to methyladenosine modification fmr phase switch rna decay, Placeholder for records without volume info and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Wang, Yongjie; Chen, Junfei; Gao, Wei-Qiang; Yang, Ru published an article.Safety of N-Methyl-7H-purin-6-amine The title of the article was METTL14 promotes prostate tumorigenesis by inhibiting THBS1 via an m6A-YTHDF2-dependent mechanism. And the article contained the following:

N6-methyladenine (m6A) is the most predominant RNA modification, which has been shown to be related to many types of cancers. However, understanding of its role in prostate cancer (PCa) is largely unknown. Here, we report an upregulation of METTL14 that was correlated with poor prognosis in PCa patients. Functionally, knocking down METTL14 inhibited tumor proliferation both in vitro and in vivo. Mech., RNA-seq and MeRIP-seq analyses identified THBS1 as the downstream target of METTL14 in PCa. METTL14 downregulated THBS1 expression in an m6A-dependent manner, which resulted in the recruitment of YTHDF2 to recognize and degrade Thrombospondin 1 (THBS1) mRNA. Thus, our findings revealed that METTL14 acted as an oncogene by inhibiting THBS1 expression via an m6A-YTHDF2-dependent manner. METTL14 could be a potential prognosis marker and a therapeutic target. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to pancreatic cancer mettl14 tumorigenesis thbs1 m6a ythdf2, Placeholder for records without volume info and other aspects.Safety of N-Methyl-7H-purin-6-amine

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Hardy, Alexis; Matelot, Melody; Touzeau, Amandine; Klopp, Christophe; Lopez-Roques, Celine; Duharcourt, Sandra; Defrance, Matthieu published an article in 2021, the title of the article was DNAModAnnot: a R toolbox for DNA modification filtering and annotation.Safety of N-Methyl-7H-purin-6-amine And the article contains the following content:

Long-read sequencing technologies can be employed to detect and map DNA modifications at the nucleotide resolution on a genome-wide scale. However, published software packages neglect the integration of genomic annotation and comprehensive filtering when analyzing patterns of modified bases detected using Pacific Biosciences (PacBio) or Oxford Nanopore Technologies (ONT) data. Here, we present DNA Modification Annotation (DNAModAnnot), a R package designed for the global anal. of DNA modification patterns using adapted filtering and visualization tools. We tested our package using PacBio sequencing data to analyze patterns of the 6-methyladenine (6mA) in the ciliate Paramecium tetraurelia, in which high 6mA amounts were previously reported. We found P. tetraurelia 6mA genome-wide distribution to be similar to other ciliates. We also performed 5-methylcytosine (5mC) anal. in human lymphoblastoid cells using ONT data and confirmed previously known patterns of 5mC. DNAModAnnot provides a tool-box for the genome-wide anal. of different DNA modifications using PacBio and ONT long-read sequencing data. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Safety of N-Methyl-7H-purin-6-amine

The Article related to deoxyribonucleic acid modification annotation r toolbox filtering, Placeholder for records without volume info and other aspects.Safety of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Qu, Shanqiang; Chen, Zhixin; Liu, Bin; Liu, Jin; Wang, Huafu published an article in 2021, the title of the article was N6-methyladenine-related genes affect biological behavior and the prognosis of glioma.Application In Synthesis of N-Methyl-7H-purin-6-amine And the article contains the following content:

Although aberrant expression of N6-methyladenine (m6A) methylation-related genes contribute to tumorigenesis in many solid tumors, the prognostic value of the m6A-related genes and their correlation with clinicopathol. features in gliomas need advanced study. Nine m6A-related genes were identified as independent prognostic factors, which were mostly enriched in RNA splicing, regulation of immune response and vesicle-mediated transport. We constructed risk score of each patient, which was highly associated with clinicopathol. features. Kaplan-Meier curve showed that the prognosis of patients with high-risk scores was significantly worse than that with low-risk scores (HR = 4.30, 95% CI = 3.16-5.85, p < 0.0001). A nomogram was constructed based on the nine m6A-related genes signature and clinicopathol. features with well-fitted calibration curves (c-index = 0.82), showing high specificity and sensitivity (area under the curve for 1-, 3-, and 5-years survival probability = 0.874, 0.918, and 0.934). A nine m6A-related genes signature was identified in gliomas. The m6A-related risk score is a novel prognostic factor for patients with glioma, and is associated with clinicopathol. features. Moreover, the nomogram based on the nine m6A-related genes signature and clinicopathol. features had good efficacy in predicting the survival probability. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application In Synthesis of N-Methyl-7H-purin-6-amine

The Article related to glioma n6 methyladenine prognosis, neoplasms, nomograms, prognosis, Placeholder for records without volume info and other aspects.Application In Synthesis of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On October 31, 2021, He, Shiqian; Kong, Liang; Chen, Jing published an article.Quality Control of N-Methyl-7H-purin-6-amine The title of the article was iDNA6mA-Rice-DL: A local web server for identifying DNA N6-methyladenine sites in rice genome by deep learning method. And the article contained the following:

Accurate detection of N6-methyladenine (6mA) sites by biochem. experiments will help to reveal their biol. functions, still, these wet experiments are laborious and expensive. Therefore, it is necessary to introduce a powerful computational model to identify the 6mA sites on a genomic scale, especially for plant genomes. In view of this, we proposed a model called iDNA6mA-Rice-DL for the effective identification of 6mA sites in rice genome, which is an intelligent computing model based on deep learning method. Traditional machine learning methods assume the preparation of the features for anal. However, our proposed model automatically encodes and extracts key DNA features through an embedded layer and several groups of dense layers. We use an independent dataset to evaluate the generalization ability of our model. An area under the receiver operating characteristic curve (auROC) of 0.98 with an accuracy of 95.96% was obtained. The experiment results demonstrate that our model had good performance in predicting 6mA sites in the rice genome. A user-friendly local web server has been established. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to rice genome web server deep learning, 6ma, dna, docker, deep learning, web server, Placeholder for records without volume info and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

On December 31, 2022, Guan, Qian; Lin, Huiran; Miao, Lei; Guo, Huiqin; Chen, Yongping; Zhuo, Zhenjian; He, Jing published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was Functions, mechanisms, and therapeutic implications of METTL14 in human cancer. And the article contained the following:

A review. RNA modification plays a crucial role in many biol. functions, and its abnormal regulation is associated with the progression of cancer. Among them, N6-methyladenine (m6A) is the most abundant RNA modification. Methyltransferase-like 14 (METTL14) is the central component of the m6A methylated transferase complex, which is involved in the dynamic reversible process of m6A modification. METTL14 acts as both an oncogene and tumor suppressor gene to regulate the occurrence and development of various cancers. The abnormal m6A level induced by METTL14 is related to tumorigenesis, proliferation, metastasis, and invasion. To date, the mol. mechanism of METTL14 in various malignant tumors has not been fully studied. In this paper, we systematically summarize the latest research progress on METTL14 as a new biomarker for cancer diagnosis and its biol. function in human tumors and discuss its potential clin. application. This study aims to provide new ideas for targeted therapy and improved prognoses in cancer. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine

The Article related to review cancer mettl14 rna modification biomarkers, cancer, drug discovery, mettl14, rna modification, m6a, Placeholder for records without volume info and other aspects.Name: N-Methyl-7H-purin-6-amine

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Gao, Chundi; Yu, Haiyang; Li, Huayao; Liu, Cun; Ma, Xiaoran; Zhuang, Jing; Sun, Changgang published an article in 2022, the title of the article was Analysis of the expression patterns and clinical relevance of m6A regulators in 33 cancer types.Quality Control of N-Methyl-7H-purin-6-amine And the article contains the following content:

The role of N6-methyladenine (m6A) RNA methylation in a variety of biol. processes is gradually being revealed. Here, we systematically describe the correlation between the expression pattern of m6A RNA methylation regulatory factors and clin. phenotype, immunity, drug sensitivity, stem cells and prognosis in more than 10,000 samples of 33 types of cancer. The results show that there are significant differences in the expression of 20 m6A RNA methylation regulatory factors in different cancers, and there was a significant correlation with the anal. indicators. In this study, the m6A RNA methylation regulatory factor was found not only to potentially assist in stratifying the prognosis but also to predict or improve the sensitivity of clin. drug therapy. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Quality Control of N-Methyl-7H-purin-6-amine

The Article related to expression pattern clin relevance regulator cancer type, n6-methyladenine, correlation, pan-cancer, regulatory factor, survival, Placeholder for records without volume info and other aspects.Quality Control of N-Methyl-7H-purin-6-amine

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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem

Li, Yin; Gu, Jie; Xu, Fengkai; Zhu, Qiaoliang; Chen, Yiwei; Ge, Di; Lu, Chunlai published an article in 2021, the title of the article was Molecular characterization, biological function, tumor microenvironment association and clinical significance of m6A regulators in lung adenocarcinoma.Recommanded Product: N-Methyl-7H-purin-6-amine And the article contains the following content:

N6-methyladenosine (m6A) modification can regulate a variety of biol. processes. However, the implications of m6A modification in lung adenocarcinoma (LUAD) remain largely unknown. Here, we systematically evaluated the m6A modification features in more than 2400 LUAD samples by analyzing the multi-omics features of 23 m6A regulators.We depicted the genetic variation features of m6A regulators, and found mutations of FTO and YTHDF3 were linked to worse overall survival. Many m6A regulators were aberrantly expressed in tumors, among which FTO, IGF2BP3, YTHDF1 and RBM15 showed consistent alteration features across 11 independent cohorts. Besides, the regulator-pathway interaction network demonstrated that m6A modification was associated with various biol. pathways, including immune-related pathways. The correlation between m6A regulators and tumor microenvironment was also assessed.We found that LRPPRC was neg. correlated with most tumor-infiltrating immune cells. On the other hand, we established a scoring tool named m6Sig, which was pos. correlated with PD-L1 expression and could ref lect both the tumor microenvironment characterization and prognosis of LUAD patients. Comparison of CNV between high and low m6Sig groups revealed differences on chromosome 7. Application of m6Sig on an anti-PD-L1 immunotherapy cohort confirmed that the high m6Sig group demonstrated therapeutic advantages and clin. benefits. Our study indicated that m6A modification is involved in many aspects of LUAD and contributes to tumor microenvironment formation. A better understanding of m6A modification will provide more insights into the mol. mechanisms of LUAD and facilitate developing more effective personalized treatment strategies. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Recommanded Product: N-Methyl-7H-purin-6-amine

The Article related to lung adenocarcinoma tumor microenvironment biol function, pd-l1 immunotherapy, lung adenocarcinoma, m6a, prognosis, tumor microenvironment, web application, Placeholder for records without volume info and other aspects.Recommanded Product: N-Methyl-7H-purin-6-amine

Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem