Ke, Mi published the artcileSpatiotemporal profiling of cytosolic signalling complexes in living cells by selective proximity proteomics, SDS of cas: 901770-40-9, the publication is Nature Communications (2021), 12(1), 71, database is CAplus and MEDLINE.
Signaling complexes are often organized in a spatiotemporal manner and on a minute timescale. Proximity labeling based on engineered ascorbate peroxidase APEX2 pioneered in situ capture of spatiotemporal membrane protein complexes in living cells, but its application to cytosolic proteins remains limited due to the high labeling background. Here, we develop proximity labeling probes with increased labeling selectivity. These probes, in combination with label-free quant. proteomics, allow exploring cytosolic protein assemblies such as phosphotyrosine-mediated protein complexes formed in response to minute-scale EGF stimulation. As proof-of-concept, we systematically profile the spatiotemporal interactome of the EGFR signaling component STS1. For STS1 core complexes, our proximity proteomics approach shows comparable performance to affinity purification-mass spectrometry-based temporal interactome profiling, while also capturing addnl.-especially endosomally-located-protein complexes. In summary, we provide a generic approach for exploring the interactome of mobile cytosolic proteins in living cells at a temporal resolution of minutes.
Nature Communications published new progress about 901770-40-9. 901770-40-9 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Benzene,Phenol,Amide, name is N-(4-Hydroxyphenyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C16H21N3O3S, SDS of cas: 901770-40-9.
Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem