Rp-hplc method development and validation for simultaneous estimation of clidinium bromide, chlordiazepoxide, dicyclomine hcl and rabeprazole sodium in pharmaceutical dosage form was written by Khan, Ummekulsum;Luhar, Shailesh V.;Narkhede, Sachin B.. And the article was included in European Journal of Biomedical and Pharmaceutical Sciences in 2018.Synthetic Route of C18H20N3NaO3S The following contents are mentioned in the article:
A simple, rapid, economical, precise and accurate RP-HPLC method for simultaneous estimation of Clidinium Bromide, Chlordiazepoxide, Dicyclomine HCl and Rabeprazole Sodium has been developed. Method was studied by using Shimadzu 2010CHT, Chromatog. separation was achieved using ODS C18 RP column (250 mm 脳 4.6 mm i.d., 5渭m) kept at ambient temperature, using a mobile phase consisting a mixture of Phosphate buffer (pH 4.5): Acetonitrile (80:20 volume/volume) and pH adjusted with 0.5% orthophosphoric acid at a flow rate of 1.0 mL/min. The detection was made at 215 nm. Retention time was 3.12 min, 4.28 min and 6.97 min and 13.29 for Clidinium Bromide, Chlordiazepoxide, Dicyclomine HCl and Rabeprazole Sodium resp. Linear correlation was obtained between peak area and concentration in the range of 5-15 渭g/mL for Clidinium Bromide, 10-30 渭g/mL for Chlordiazepoxide, 20-60 渭g/mL for Dicyclomine HCl and Rabeprazole Sodium. The percentage recoveries of four drugs Clidinium Bromide, Chlordiazepoxide, Dicyclomine HCl and Rabeprazole Sodium were found to be 100.48-101.46 %, 98.87-101.75%,98.20-98.80% and 100.94-101.71 % resp. The % RSD value was less than 2, for intraday and interday precision. It can be successfully used for routine anal. of Clidinium Bromide, Chlordiazepoxide, Dicyclomine HCl and Rabeprazole Sodium in combined solid dosage form without any interference from common excipients and impurity. This study involved multiple reactions and reactants, such as Sodium 2-(((4-(3-methoxypropoxy)-3-methylpyridin-2-yl)methyl)sulfinyl)benzo[d]imidazol-1-ide (cas: 117976-90-6Synthetic Route of C18H20N3NaO3S).
Sodium 2-(((4-(3-methoxypropoxy)-3-methylpyridin-2-yl)methyl)sulfinyl)benzo[d]imidazol-1-ide (cas: 117976-90-6) belongs to imidazole derivatives. Imidazole derivatives generally have good solubility in protic solvents. Simple imidazole derivatives, such as 1H-imidazole, 2-methyl-1H-imidazole, and 1,2-dimethylimidazole, have very high solubility in water. Imidazole also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes.Synthetic Route of C18H20N3NaO3S
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem