On January 31, 2020, Zhang, Shuming; Li, Bianbian; Du, Ke; Liang, Tingting; Dai, Mengyuan; Huang, Wenxin; Zhang, Huizhi; Ling, Yihui; Zhang, Huidong published an article.Application of 443-72-1 The title of the article was Epigenetically modified N6-methyladenine inhibits DNA replication by human DNA polymerase iota. And the article contained the following:
Among human four Y-family DNA polymerases, hPol ι is exceptionally error-prone in DNA synthesis. 6 mA plays significant roles in epigenetic regulation of numerous biol. processes. Nonetheless, its effects on DNA replication by hPol ι is still unclear. In this work, we found that 6 mA and Hyp, the intermediate of 6 mA, inhibited the replication of DNA by hPol ι. 6 mA lost priority in extension beyond 6 mA:T pair, partially reducing dTTP incorporation efficiency and inhibiting next-base extension. Hyp was prone to dCTP incorporation and extension beyond Hyp:C instead of Hyp:T pair. Statistically, 6 mA primarily reduced the burst incorporation rate (kpol) and slightly increased the dissociation constant (Kd,dTTP). However, Hyp mainly increased the Kd,dCTP yet did not affect the kpol, both reducing the burst incorporation efficiency (kpol/Kd,dCTP). 6 mA together with Hyp weakened the binding affinity of hPol ι to DNA in binary or ternary complex. The misincorporation opposite 6 mA or Hyp further weakened this binding affinity. The Me group in 6 mA doesn′t almost affect the H-bond formation with dTTP, therefore mildly inhibiting dTTP incorporation. As an analog of G, Hyp can form only two H-bonds with dCTP, thus reducing dCTP incorporation. This work provides a new insight in how the epigenetically modified 6 mA and its intermediate Hyp affect replication of DNA by human DNA polymerase ι. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1
The Article related to dna polymerase, dna replication, human dna polymerase ι, hypoxanthine (hyp), n(6)-methyladenine (6 ma), steady-state and pre-steady-state kinetics, and other aspects.Application of 443-72-1
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem