On June 30, 2021, Zhao, Peng; Ren, Shi-Miao; Liu, Feng; Zheng, Yu-Cong; Xu, Na; Pan, Jiang; Yu, Hui-Lei; Xu, Jian-He published an article.Related Products of 73590-85-9 The title of the article was Protein engineering of thioether monooxygenase to improve its thermostability for enzymatic synthesis of chiral sulfoxide. And the article contained the following:
Esomeprazole, the S-enantiomer of omeprazole, is the best-selling proton pump inhibitor. In our previous work, a mutant of cyclohexanone monooxygenase from the Acinetobacter calcoaceticus (named AcCHMO-M6) was successfully obtained through protein engineering which could catalyze the oxidation of omeprazole sulfide. However, its practical application is still hindered by the poor thermostability, especially in the up-scaled reaction process. In this work, site mutagenesis based on consensus anal. and directed evolution were used to engineer this enzyme in order to improve the stability of AcCHMO-M6. The half-lives of the resultant mutants AcCHMO-M9 (F29L/R444E) and AcCHMO-M10 (F29L/R444E/A145S/G430T) at 40°C were increased from 2.2 h to 8.5 h and 5.9 h resp., while the corresponding Tm values were increased by 7°C and 5.3°C in comparison to AcCHMO-M6. The specific activity of AcCHMO-M9 was comparable to that of AcCHMO-M6, and the specific activity of AcCHMO-M10 was about 4-fold that of AcCHMO-M6. The AcCHMO-M10 catalyzed sulfide oxidation reaction reached 100% conversion after 16 h at 30°C, in contrast to 39.4% conversion in the case of AcCHMO-M6. These results show that the potential of this thioether monooxygenase can be significantly improved by protein engineering. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).Related Products of 73590-85-9
The Article related to acinetobacter cyclohexanone monooxygenase protein engineering thermostability chiral sulfoxide, Fermentation and Bioindustrial Chemistry: Industrial Chemicals and other aspects.Related Products of 73590-85-9
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem