Tian, Meng; Li, Zhenhua; Song, Ruihong; Li, Yingxian; Guo, Chengang; Sha, Yujie; Cui, Wanling; Xu, Shicai; Hu, Guodong; Wang, Jihua published the artcile< Graphene biosensor as affinity biosensors for biorecognition between Guanine riboswitch and ligand>, Electric Literature of 452-06-2, the main research area is graphene field effect transistor biosensor guanine riboswitch ligand.
Guanine riboswitch (GR) is located in the untranslated region of mRNA that performs important biol. functions by interactions with specific ligand mols. The present standard methods for detecting mRNA has been widely used, but suffer from extra labeling steps, high cost, low throughput, low sensitivities and limited dynamic ranges. The field effect transistor (FET) biosensors showed high performance in detecting a large number of biol. analytes. However, the detection of analytes with low charge and small mol. still remains a challenge. In this paper, a label-free graphene field effect transistor (G-FET) biosensor has been developed to detect the binding interactions between GR and four purine analog ligand mols. (GUA, 6GU, 2BP, XAN). We demonstrated that the G-FET biosensor were highly sensitive in detecting the elec.-neutral and low mol. weight ligands by using GR as probe. The sensing mechanisms for elec.-neutral mol. is revealed by mol. dynamics simulation. The equilibrium dissociation constants KD and binding free energy ΔG of GR-GUA, GR-6GU, GR-2BP and GR-XAN were obtained by the G-FET biosensor, and the results are comparable with the calculated by mol. dynamics simulation and previous reports. We demonstrated that the G-FET biosensor can be used as affinity biosensors to quantify biomol. interactions.
Applied Surface Science published new progress about Aptamers (guanine riboswitch). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Electric Literature of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem