Guillen, Danielle; Schievelbein, Mika; Patel, Kushkumar; Jose, Davis; Ouellet, Jonathan published the artcile< A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining>, Product Details of C5H5N5, the main research area is nucleic acid SYBR gold gel staining kinetic assay.
Labeling substrates or products are paramount in determining enzymic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity. However, those methods have their own drawbacks such as half-life decay, expensive and hazardous. Here, we propose a novel, simple, economical and fast alternative to substrate labeling for studying the kinetics of nucleic acids: post-migration gel staining with SYBR Gold. Cleavage rates similar to the ones reported in the literature for the I-R3 DNA-cleaving DNA enzyme in the presence of zinc chloride are an indication of the quality of the new method. Moreover, the activity of the hammerhead ribozyme was also monitored by our method to illustrate its versatility. This labeling-free method has several advantages such as its ease of use as well as cost effective and versatility with both non-structured and structured RNAs or DNAs.
PLoS One published new progress about Biological staining. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Product Details of C5H5N5.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem