Kamiya, Yukiko; Sato, Fuminori; Murayama, Keiji; Kodama, Atsuji; Uchiyama, Susumu; Asanuma, Hiroyuki published the artcile< Incorporation of Pseudo-complementary Bases 2,6-Diaminopurine and 2-Thiouracil into Serinol Nucleic Acid (SNA) to Promote SNA/RNA Hybridization>, Quality Control of 452-06-2, the main research area is diaminopurine thiouracil serinol nucleic acid RNA hybridization; 2,6-diaminopurine; 2-thiouracil; acyclic nucleic acid; pseudo complementary base; serinol nucleic acid.
Serinol nucleic acid (SNA) is a promising candidate for nucleic acid-based mol. probes and drugs due to its high affinity for RNA. The authors’ previous work revealed that incorporation of 2,6-diaminpurine (D), which can form three hydrogen bonds with uracil, into SNA increases the melting temperature of SNA-RNA duplexes. However, D incorporation into short self-complementary regions of SNA promoted self-dimerization and hindered hybridization with RNA. Here the authors synthesized a SNA monomer of 2-thiouracil (sU), which was expected to inhibit base pairing with D by steric hindrance between sulfur and the amino group. To prepare the SNA containing D and sU in high yield, the authors customized the protecting groups on D and sU monomers that can be readily deprotected under acidic conditions. Incorporation of D and sU into SNA facilitated stable duplex formation with target RNA by suppressing the self-hybridization of SNA and increasing the stability of the heteroduplex of SNA and its complementary RNA. The authors’ results have important implications for the development of SNA-based probes and nucleic acid drugs.
Chemistry – An Asian Journal published new progress about Nucleic acid hybridization (SNA-RNA). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Quality Control of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem