Application of 7720-39-0In 2017 ,《insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex》 was published in Proceedings of the National Academy of Sciences of the United States of America. The article was written by Zhang, Wen; Tam, Chun Pong; Walton, Travis; Fahrenbach, Albert C.; Birrane, Gabriel; Szostak, Jack W.. The article contains the following contents:
The nonenzymic copying of RNA templates with imidazole-activated nucleotides is a well-studied model for the emergence of RNA self-replication during the origin of life. We have recently discovered that this reaction can proceed through the formation of an imidazolium-bridged dinucleotide intermediate that reacts rapidly with the primer. To gain insight into the relationship between the structure of this intermediate and its reactivity, we cocrystd. an RNA primer-template complex with a close analog of the intermediate, the triphosphate-bridged guanosine dinucleotide GpppG, and solved a high-resolution X-ray structure of the complex. The structure shows that GpppG binds the RNA template through two Watson-Crick base pairs, with the primer 3′-hydroxyl oriented to attack the 5′-phosphate of the adjacent G residue. Thus, the GpppG structure suggests that the bound imidazolium-bridged dinucleotide intermediate would be preorganized to react with the primer by in-line SN2 substitution. The structures of bound GppG and GppppG suggest that the length and flexibility of the 5′-5′ linkage are important for optimal preorganization of the complex, whereas the position of the 5′-phosphate of bound pGpG explains the slow rate of oligonucleotide ligation reactions. Our studies provide a structural interpretation for the observed reactivity of the imidazolium-bridged dinucleotide intermediate in nonenzymic RNA primer extension. In the part of experimental materials, we found many familiar compounds, such as 1H-Imidazol-2-amine(cas: 7720-39-0Application of 7720-39-0)
1H-Imidazol-2-amine(cas: 7720-39-0) belongs to anime. To avoid the problem of multiple alkylation, methods have been devised for “blocking” substitution so that only one alkyl group is introduced. The Gabriel synthesis is one such method; it utilizes phthalimide, C6H4(CO)2NH, whose one acidic hydrogen atom has been removed upon the addition of a base such as KOH to form a salt.Application of 7720-39-0
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem