Li, Yuqing; Liu, Juewen published the artcile< Highly Specific Recognition of Guanosine Using Engineered Base-Excised Aptamers>, Related Products of 452-06-2, the main research area is guanosine base excised aptamers sodium secondary structure; DNA structures; aptamers; biosensors; fluorescence; nucleobases.
Purines and their derivatives are highly important mols. in biol. for nucleic acid synthesis, energy storage, and signaling. Although many DNA aptamers have been obtained for binding adenine derivatives such as adenosine, adenosine monophosphate, and ATP, success for the specific binding of guanosine has been limited. Instead of performing new aptamer selections, we report herein a base-excision strategy to engineer existing aptamers to bind guanosine. Both a Na+-binding aptamer and the classical adenosine aptamer have been manipulated as base-excising scaffolds. A total of seven guanosine aptamers were designed, of which the G16-deleted Na+ aptamer showed the highest bindng specificity and affinity for guanosine with an apparent dissociation constant of 0.78 mM. Single monophosphate difference in the target mol. was also recognizable. The generality of both the aptamer scaffold and excised site were systematically studied. Overall, this work provides a few guanosine binding aptamers by using a non-SELEX method. It also provides deeper insights into the engineering of aptamers for mol. recognition.
Chemistry – A European Journal published new progress about DNA aptamers Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Related Products of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem