Radhakrishnan, Harikrishnan; Javitz, Harold S.; Bhatnagar, Parijat published the artcile< Lentivirus Manufacturing Process for Primary T-Cell Biofactory Production>, Recommanded Product: 7H-Purin-2-amine, the main research area is FRalpha HDAC CAR T cell growth viral infection; CAR T cells; cell engineering; cell manufacturing; drug delivery; high-titer lentivirus production.
A process for maximizing the titer of lentivirus particles, deemed to be a necessity for transducing primary cells, is developed. Lentivirus particles, with a set of transgenes encoding an artificial cell-signaling pathway, are used to transform primary T cells as vectors for calibrated synthesis of desired proteins in situ, i.e., T-cell biofactory cells. The process is also used to generate primary T cells expressing antigen-specific chimeric antigen receptors, i.e., CAR T cells. The two differently engineered primary T cells are expanded and validated for their resp. functions, i.e., calibrated synthesis of desired proteins upon engaging the target cells, which is specific for the T-cell biofactory cells, and cytolysis of the target cells common to both types of cells. The process is compliant with current Good Manufacturing Practices and can be used to support the scale-up for clin. translation.
Advanced Biosystems published new progress about Biomarkers. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Recommanded Product: 7H-Purin-2-amine.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem