Ghosh, Tonmoy; Mondal, Aniruddha; Vamsi Bharadwaj, S. V.; Mishra, Sandhya published the artcile< A naturally fluorescent protein C-phycoerythrin and graphene oxide bio-composite as a selective fluorescence ""turn off/on"" probe for DNA quantification and characterization>, Recommanded Product: 7H-Purin-2-amine, the main research area is C phycoerythrin graphene oxide DNA quantification fluorescence characterization; C-phycoerythrin; DNA sensing; Fluorescence quenching; Graphene oxide; Protein – graphene oxide interaction.
Highly specific graphene-DNA interactions have been at the forefront of graphene-based sensor design for various analytes, including DNA itself. However, in addition to its detection, DNA also needs to be characterized according to its size and concentration in a sample, which is an addnl. anal. step. Designing a highly sensitive and selective DNA sensing and characterization platform is, thus, of great interest. The present study demonstrates that a bio-derived, naturally fluorescent protein C-phycoerythrin (CPE) – graphene oxide (GO) bio-composite can be used to detect dsDNA in nanomolar quantities efficiently via fluorescent “”turn off/on”” mechanism. Interaction with GO temporarily quenches CPE fluorescence in a dose-dependent manner. Anal. characterization indicates an indirect charge transfer with a corresponding loss of crystalline GO structure. The fluorescence is regained with the addition of DNA, while other biomols. do not pose any hinderance in the detection process. The extent of regain is DNA length dependent, and the corresponding calibration curve successfully quantifies the size of an unknown DNA. The incubation time for detection is ∼3-5 min. The bio-composite platform also works successfully in a complex biomol. matrix and cell lysate. However, the presence of serum albumin poses a hinderance in the serum sample. Particle size anal. proves that CPE displacement from GO surface by the incoming DNA is the reason for the “”turn on”” response, and that the sensing process is exclusive to dsDNA. This new platform could be an exciting and rapid DNA sensing and characterization tool.
International Journal of Biological Macromolecules published new progress about Albumins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Recommanded Product: 7H-Purin-2-amine.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem