Development of validated stability indicating RP-HPLC method for simultaneous estimation of amlodipine besylate and candesartan cilexetil from tablet was written by Padole, Y. F.;Rabade, S. K.;Jirvankar, P. S.;Umekar, M. J.;Lohiya, R. T.. And the article was included in World Journal of Pharmaceutical Research in 2021.Category: imidazoles-derivatives This article mentions the following:
A simple, isocratic, rapid and accurate reversed phase high performance liquid chromatog. method was developed for the quant. determination of Candesartan Cilexetil and Amlodipine Besylate tablets. The chromatog. separation was achieved on Water Xterra R18, 150×4.6 mm, 3.5u (C18) using Mobile Phase A : ACN: Water: OPA (950:50:01) and Mobile Phase B: ACN: Water: OPA (50:950:01), and the 位max of Amlodipine Besylate was detected at 237nm, where Candesartan Cilexetil exhibits sufficient absorbance at 254 nm. The linear range for Candesartan Cilexetil and Amlodipine Besylate were (2.8-42ppm) and (6.4-96) was obtained with correlation coefficients 鈮?.999 for each analyte. The retention time were found to be 4.2 and 8.5 min Candesartan Cilexetil and Amlodipine Besylate resp. Candesartan Cilexetil and Amlodipine Besylate was subjected to stress conditions (hydrolysis (acid, base) oxidation, photolysis, thermal degradation and humidity degradation) and the stressed samples were analyzed by use of the method. The major degradation was observed in base and minor in acid, thermal, oxidation, humidity and photolysis. The forced degradation studies prove the stability indicating power of the method. In the experiment, the researchers used many compounds, for example, 1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate (cas: 145040-37-5Category: imidazoles-derivatives).
1-(((Cyclohexyloxy)carbonyl)oxy)ethyl 1-((2′-(2H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl)methyl)-2-ethoxy-1H-benzo[d]imidazole-7-carboxylate (cas: 145040-37-5) belongs to imidazole derivatives. Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar. Imidazole has been usedin the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein, in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography, as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells.Category: imidazoles-derivatives
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem