Amino acids. I. Preparation and properties of glycocyamidine was written by McKay, A. F.;Braun, R. O.;Hatton, W. G.. And the article was included in Canadian Journal of Chemistry in 1954.COA of Formula: C6H8N2O2S This article mentions the following:
Free guanidine with Et glycinate gave 29% glycocyamidine (I). Heating guanidine carbonate with glycine produced no I, but 55% guanidinoacetic acid (II). This was esterified with EtOH by azeotropic removal of H2O by C6H6 from an alc. HCl solution to produce Et guanidinoacetate-HCl (III); picrate, m. 189.5-90.5掳. Aqueous III treated with Amberlite IRA-400 and the resulting solution evaporated gave a mixture of I (identified as its picrate, m. 214.5-15.5掳) and II. I was nitrated in Ac2O-HNO3 to glycocyamidine nitrate, m. 145掳 (from EtOH). I and II may be separated and identified by paper chromatog. with BuOH-H2O-HOAc (4.2:4.2:1) as eluent. In the experiment, the researchers used many compounds, for example, Ethyl 2-mercapto-1H-imidazole-4-carboxylate (cas: 64038-64-8COA of Formula: C6H8N2O2S).
Ethyl 2-mercapto-1H-imidazole-4-carboxylate (cas: 64038-64-8) belongs to imidazole derivatives. Many natural products, especially alkaloids, contain the imidazole ring. These imidazoles share the 1,3-C3N2 ring but feature varied substituents. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).COA of Formula: C6H8N2O2S
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem