Yu, Hye-Weon published the artcileMultiplex competitive microbead-based flow cytometric immunoassay using quantum dot fluorescent labels, Quality Control of 359860-27-8, the publication is Analytica Chimica Acta (2012), 191-198, database is CAplus and MEDLINE.
The simultaneous anal. of environmental hazards, microcarrier-based multiplex technologies show great promise. Further integration with biofunctionalized quantum dots (QDs) creates new opportunities to extend the capabilities of multicolor flow cytometry with their unique fluorescence properties. We developed a competitive microbead-based flow cytometric immunoassay using QDs fluorescent labels for simultaneous detection of 2 analytes, bringing the benefits of sensitive, rapid and easy-of-manipulation anal. tool for environmental contaminants. As model target compounds, the cyanobacterial toxin microcystin-LR and the polycyclic aromatic hydrocarbon compound benzo[a]pyrene were selected. The assay was carried out in 2 steps: the competitive immunol. reaction of multiple targets using their exclusive sensing elements of QD/antibody detection probes and antigen-coated microsphere, and the subsequent flow cytometric anal. The fluorescence of the QD-encoded microsphere was thus found to be inversely proportional to target analyte concentration Under optimized conditions, the proposed assay performed well within 30 min for the identification and quant. anal. of the 2 contaminants. For microcystin-LR and benzo[a]pyrene, dose-response curves with IC50 values of 5 and 1.1 μg/L and dynamic ranges of 0.52-30 and 0.13-10 μg/L were obtained, resp. Recovery was 92.6-106.5% for 5 types of water samples (bottled water, tap water, surface water and seawater) using only filtration as sample pretreatment.
Analytica Chimica Acta published new progress about 359860-27-8. 359860-27-8 belongs to imidazoles-derivatives, auxiliary class Other Aliphatic Heterocyclic,Chiral,Amine,Amide,Ether,Inhibitor, name is N-(2-(2-(2-(2-Aminoethoxy)ethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide, and the molecular formula is C13H9FO, Quality Control of 359860-27-8.
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https://en.wikipedia.org/wiki/Imidazole,
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