Gerlinger, Erich published the artcileMechanistic studies of the urocanase reaction using proton and phosphorus-31 NMR spectroscopy and the substrate analog 2-methylurocanate, Category: imidazoles-derivatives, the publication is Tetrahedron (1983), 39(21), 3523-8, database is CAplus.
The reaction of 2-methylurocanate with urocanase from Pseudomonas putida was monitored by 1H NMR spectroscopy at 500 MHz. 2-Methylurocanate reacted 128-fold more slowly with urocanase than did urocanate. No signals for the enol form of the 2-methylimidazolone propionate produced were detected. 2-Methylimidazolone propionate was ∼25-fold more stable to hydrolysis than imidazolone propionate. The urocanase-catalyzed exchange of the 5-H of 2-methylurocanate with the solvent 2H was 1.3-fold faster than the overall reaction. The nonenzymic exchange of the Me protons of 2-methylimidazolone propionate with solvent 2H took place with a half-life of 5.8 h. 1H NMR spectroscopy showed that the urocanase reaction is reversible. At 8° and pD 6.3, 1.6% of the total imidazolone propionate was converted into urocanate. Apart from the pyrophosphate ester group of NAD, no phosphorylated groups were detected in urocanase by 31P NMR spectroscopy.
Tetrahedron published new progress about 45533-87-7. 45533-87-7 belongs to imidazoles-derivatives, auxiliary class Imidazole,Alcohol,Imidazole, name is (2-Methyl-1H-imidazol-4-yl)methanol, and the molecular formula is C5H8N2O, Category: imidazoles-derivatives.
Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem