Dindar, Cigdem K. published the artcileThe Development of Spectrophotometric and Validated Stability- Indicating RP-HPLC Methods for Simultaneous Determination of Ephedrine HCL, Naphazoline HCL, Antazoline HCL, and Chlorobutanol in Pharmaceutical Pomade Form, Synthetic Route of 2508-72-7, the publication is Combinatorial Chemistry & High Throughput Screening (2020), 23(10), 1090-1099, database is CAplus and MEDLINE.
Allergic rhinitis, acute nasal congestion and sinusitis are one of the most common health problems and have a major effect on the quality of life. Several medications are used to improve the symptoms of such diseases in humans. Pharmaceutical pomade form containing Ephedrine (EPD) HCl, Naphazoline (NPZ) HCl, Antazoline (ANT) HCl, and Chlorobutanol (CLO) is one of them. Objective: For these reasons, this study includes the development of spectrophotometric and chromatog. methods for the determination of EPD HCl, NPZ HCl, ANT HCl, and CLO active agents in the pharmaceutical pomade. Method: In the spectrophotometric method, third-order derivative of the amplitudes at 218 nm n=5 and the first-order derivative of the amplitudes 254 nm n=13 was selected for the determination of EPD, ANT, resp. while NPZ was determined by the second derivative at 234 nm and n=21. Colorimetric detection was applied for assay anal. of CLO at 540 nm. Furthermore, a reverse phase high performance liquid chromatog. (RP- HPLC) method has been developed and optimized by using Agilent Zorbax Eclipse XDB C18 (75 mm x 3.0 mm, 3.5μm) column. The column temperature was 40°C, binary gradient elution was used and the mobile phase consisted of 15 mM phosphate buffer in distilled water (pH 3.0) and methanol, and the flow rate was 0.6 mL min-1 and the UV detector was detected at 210 nm. The linear operating range was obtained as 11.97-70, 0.59-3, 2.79-30, and 2.92-200μg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO resp. The LOD values were found to be 3.95, 0.19, 0.92 and 0.96μg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO in the spectrophotometric method, resp. The linear ranges in the RP-HPLC method were 8.2-24.36μg mL-1, 0.083 – 0.75μg/mL, 2.01-7.5μg mL-1 and 2.89-24.4μg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO, resp. The LOD values in the validation studies were 2.7, 0.025, 0.66 and 0.86μg mL-1 for EPD HCl, NPZ HCl, ANT HCl, and CLO in RP-HPLC method resp. Conclusion: The results of the spectrophotometric and chromatog. methods were compared and no differences were found between the two methods.
Combinatorial Chemistry & High Throughput Screening published new progress about 2508-72-7. 2508-72-7 belongs to imidazoles-derivatives, auxiliary class Inhibitor,Immunology/Inflammation,Histamine Receptor, name is N-Benzyl-N-((4,5-dihydro-1H-imidazol-2-yl)methyl)aniline hydrochloride, and the molecular formula is C17H20ClN3, Synthetic Route of 2508-72-7.
Referemce:
https://en.wikipedia.org/wiki/Imidazole,
Imidazole | C3H4N2 – PubChem