On October 22, 1999, Lutsenko, Eugene; Bhagwat, Ashok S. published an article.Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was The role of the Escherichia coli Mug protein in the removal of uracil and 3,N4-ethenocytosine from DNA. And the article contained the following:
The human thymine-DNA glycosylase has a sequence homolog in Escherichia coli that is described to excise uracils from U路G mismatches (Gallinari, P., and Jiricny, J. (1996) Nature 383, 735-738) and is named mismatched uracil glycosylase (Mug). It has also been described to remove 3,N4-ethenocytosine (蔚C) from 蔚C路G mismatches (Saparbaev, M., and Laval, J. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 8508-8513). We used a mug mutant to clarify the role of this protein in DNA repair and mutation avoidance. We find that inactivation of mug has no effect on C to T or 5-methylcytosine to T mutations in E. coli and that this contrasts with the effect of ung defect on C to T mutations and of vsr defect on 5-methylcytosine to T mutations. Even under conditions where it is overproduced in cells, Mug has little effect on the frequency of C to T mutations. Because uracil-DNA glycosylase (Ung) and Vsr are known to repair U路G and T路G mismatches, resp., we conclude that Mug does not repair U路G or T路G mismatches in vivo. A defect in mug also has little effect on forward mutations, suggesting that Mug does not play a role in avoiding mutations due to endogenous damage to DNA in growing E. coli. Cell-free extracts from mug+ ung cells show very little ability to remove uracil from DNA, but can excise 蔚C. The latter activity is missing in extracts from mug cells, suggesting that Mug may be the only enzyme in E. coli that can remove this mutagenic adduct. Thus, the principal role of Mug in E. coli may be to help repair damage to DNA caused by exogenous chem. agents such as chloroacetaldehyde. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to escherichia dna repair mismatched uracil glycosylase mug protein ethenocytosine, General Biochemistry: Subcellular Processes and other aspects.Quality Control of Imidazo[1,2-c]pyrimidin-5(6H)-one
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Imidazole | C3H4N2 – PubChem