On April 20, 1993, Palejwala, Vaseem A.; Rzepka, Robert W.; Simha, Devendranath; Humayun, M. Zafri published an article.Recommanded Product: 55662-66-3 The title of the article was Quantitative multiplex sequence analysis of mutational hot spots. Frequency and specificity of mutations induced by a site-specific ethenocytosine in M13 viral DNA. And the article contained the following:
An assay is described for determining the frequency and specificity of mutations occurring at hot spots within a population of DNA mols. The procedure consists of (a) annealing the DNA population with a labeled oligonucleotide designed to prime DNA synthesis at the mutational hot spot; (b) DNA elongation in the presence of a single dideoxynucleoside triphosphate together with 1-3 deoxynucleoside triphosphates, and (c) quantitation of all limit elongation products by high-resolution gel electrophoresis followed by autoradiog. and computing densitometry. Derivation of mutational frequency and specificity over a wide range of values is demonstrated for M13 viral DNA mixtures containing defined proportions of wild-type and mutant DNAs, as well as for M13 viral DNA populations obtained by transfection of DNA bearing a defined site-specific ethenocytosine lesion. The assay is shown to yield results similar to those obtained by laborious clone-by-clone sequencing of viral progeny. The method is not affected significantly by several tested variables and appears to be suitable for use as a quant. assay for sequence microheterogeneity at defined positions within DNA populations. Application of the methodol. demonstrates that ethenocytosine, an exocyclic DNA lesion induced by carcinogens such as vinyl chloride and urethane, is a highly efficient mutagenic lesion with a mutational specificity expected for noninstructive lesions. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: 55662-66-3
The Article related to mutation hot spot multiplex sequence analysis, m13 virus dna mutation ethenocytosine specificity, Biochemical Genetics: Methods and other aspects.Recommanded Product: 55662-66-3
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