On July 23, 2020, Li, Zheng; Zhao, Shuai; Nelakanti, Raman V.; Lin, Kaixuan; Wu, Tao P.; Alderman, Myles H. III; Guo, Cheng; Wang, Pengcheng; Zhang, Min; Min, Wang; Jiang, Zongliang; Wang, Yinsheng; Li, Haitao; Xiao, Andrew Z. published an article.COA of Formula: C6H7N5 The title of the article was N6-methyladenine in DNA antagonizes SATB1 in early development. And the article contained the following:
Abstract: The recent discovery of N6-methyladenine (N6-mA) in mammalian genomes suggests that it may serve as an epigenetic regulatory mechanism1. However, the biol. role of N6-mA and the mol. pathways that exert its function remain unclear. Here we show that N6-mA has a key role in changing the epigenetic landscape during cell fate transitions in early development. We found that N6-mA is upregulated during the development of mouse trophoblast stem cells, specifically at regions of stress-induced DNA double helix destabilization (SIDD)2-4. Regions of SIDD are conducive to topol. stress-induced unpairing of the double helix and have critical roles in organizing large-scale chromatin structures3,5,6. We show that the presence of N6-mA reduces the in vitro interactions by more than 500-fold between SIDD and SATB1, a crucial chromatin organizer that interacts with SIDD regions. Deposition of N6-mA also antagonizes SATB1 function in vivo by preventing its binding to chromatin. Concordantly, N6-mA functions at the boundaries between euchromatin and heterochromatin to restrict the spread of euchromatin. Repression of SIDD-SATB1 interactions mediated by N6-mA is essential for gene regulation during trophoblast development in cell culture models and in vivo. Overall, our findings demonstrate an unexpected mol. mechanism for N6-mA function via SATB1, and reveal connections between DNA modification, DNA secondary structures and large chromatin domains in early embryonic development. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).COA of Formula: C6H7N5
The Article related to methyladenine dna methylation satb1 embryogenesis mouse trophoblast, Biochemical Genetics: Genomic Processes and other aspects.COA of Formula: C6H7N5
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem