On December 2, 2004, Jurado, Juan; Maciejewska, Agnieszka; Krwawicz, Joanna; Laval, Jacques; Saparbaev, Murat K. published an article.Safety of Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Role of mismatch-specific uracil-DNA glycosylase in repair of 3,N4-ethenocytosine in vivo. And the article contained the following:
The 3,N4-ethenocytosine (εC) residue might have biol. role in vivo since it is recognized and efficiently excised in vitro by the E. coli mismatch-specific uracil-DNA glycosylase (MUG) and the human thymine-DNA glycosylase (hTDG). In the present work we have generated mug defective mutant of E. coli by insertion of a kanamycin cassette to assess the role of MUG in vivo. We show that human TDG complements the enzymic activity of MUG when expressed in a mug mutant. The εC-DNA glycosylase defective strain did not exhibit spontaneous mutator phenotype and did not show unusual sensitivity to any of the following DNA damaging treatments: methylmethanesulfonate, N-methyl-N’-nitro-N-nitrosoguanidine, UV light, H2O2, paraquat. However, plasmid DNA damaged by 2-chloroacetaldehyde treatment in vitro was inactivated at a greater rate in a mug mutant than in wild-type host, suggesting that MUG is required for the in vivo processing of the ethenobases. In addition, 2-chloroacetaldehyde treatment induces preferentially G·C → C·G and A·T → T·A transversions in mug mutant. Comparison of the mutation frequencies induced by the site-specifically incorporated εC residue in E. coli wild-type vs. mug indicates that MUG repairs more than 80% of εC residues in vivo. Furthermore, the results show that nucleotide excision repair and recombination are not involved in the processing of εC in E. coli. Based on the mutagenesis data we suggest that εC may be less toxic and less mutagenic than expected. The increased spontaneous mutation rate for G·C → A·T transition in the ung mug double mutant as compared to the single ung mutant suggest that MUG may be a back-up repair enzyme to the classic uracil-DNA glycosylase. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Safety of Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to uracil dna glycosylase repair ethenocytosine escherichia, Biochemical Genetics: Genomic Processes and other aspects.Safety of Imidazo[1,2-c]pyrimidin-5(6H)-one
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem