On September 10, 1991, Simha, Devendranath; Palejwala, Vaseem A.; Humayun, M. Zafri published an article.Related Products of 55662-66-3 The title of the article was Mechanisms of mutagenesis by exocyclic DNA adducts. Construction and in vitro template characteristics of an oligonucleotide bearing a single site-specific ethenocytosine. And the article contained the following:
It is widely accepted that mutagenic DNA lesions fall into two categories: mispairing lesions hydrogen bond with an incorrect incoming base, generally do not stop replication, and possess high mutagenic efficiency without any requirement for induced functions; noninstructional lesions lack accessible template information, act as strong blocks to DNA replication (and are therefore toxic), and their mutagenic effects are SOS-dependent. The recent results show that a noninstructional exocyclic DNA lesion induced by vinyl chloride, may have unusual mutagenic properties. To obtain more definitive exptl. evidence for the observed effects, a single ethenocytosine (εC) residue was introduced at a specific site of coliphage M13AB28 replicative form DNA by a single-stranded linker-ligation technique. The resulting DNA was purified and transfected into appropriate recA+ or recA- Escherichia coli host cells. The effect of εC on survival was determined from transfection efficiency. Both the frequency and specificity of mutations induced by εC were determined by direct sequence anal. of randomly picked progeny phage plaques. The results indicated that εC has little effect on the survival of M13 DNA. Approx. 30% of the progeny phage obtained by transfecting εC DNA had a base substitution mutation precisely at the lesion site. No such mutations were observed in progeny plaques obtained by transfecting the control DNA construct. All εC-induced mutations were either C-to-T transitions or C-to-A transversions. Neither survival nor mutagenic efficiency was significantly affected in recA- host cells. The findings reported here and in the preceding paper suggest that ethenocytosine may represent a novel type of RecA-independent, highly mutagenic noninstructional DNA lesion. These and other results argue that a requirement for SOS functions is neither a defining attribute nor an exclusive attribute of noninstructional lesions. These data also support the possibility that mutation recovery (i.e., lesion bypass) rather than misincorporation may be the major role of SOS functions in mutagenesis. Finally, the high mutagenic efficiency observed makes εC a reasonable candidate for mediating the genotoxic properties of vinyl chloride, a suspected human carcinogen, and a major industrial chem. that is produced in large quantities around the world. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Related Products of 55662-66-3
The Article related to ethenocytosine lesion dna mutagenicity, Toxicology: Carcinogens, Mutagens, and Teratogens and other aspects.Related Products of 55662-66-3
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