Yang, Zuxiao; Wang, Jiayin; Huang, Lin; Lilley, David M. J.; Ye, Keqiong published an article in 2020, the title of the article was Functional organization of box C/D RNA-guided RNA methyltransferase.HPLC of Formula: 443-72-1 And the article contains the following content:
Box C/D RNA protein complexes (RNPs) catalyze site-specific 2′-O-methylation of RNA with specificity determined by guide RNAs. In eukaryotic C/D RNP, the paralogous Nop58 and Nop56 proteins specifically associate with terminal C/D and internal C’/D’ motifs of guide RNAs, resp. We have reconstituted active C/D RNPs with recombinant proteins of the thermophilic yeast Chaetomium thermophilum. Nop58 and Nop56 could not distinguish between the two C/D motifs in the reconstituted enzyme, suggesting that the assembly specificity is imposed by trans-acting factors in vivo. The two C/D motifs are functionally independent and halfmer C/D RNAs can also guide site-specific methylation. Extensive pairing between C/D RNA and substrate is inhibitory to modification for both yeast and archaeal C/D RNPs. N6-methylated adenine at box D/D interferes with the function of the coupled guide. Our data show that all C/D RNPs share the same functional organization and mechanism of action and provide insight into the assembly specificity of eukaryotic C/D RNPs. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).HPLC of Formula: 443-72-1
The Article related to box cd rna methyltransferase n6 methylation protein complexe, Biochemical Genetics: Gene Structure and Organization and other aspects.HPLC of Formula: 443-72-1
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem