On January 10, 2020, Wang, Baihui; Luo, Qiang; Li, Yingping; Yin, Liufan; Zhou, Nana; Li, Xiangnan; Gan, Jianhua; Dong, Aiwu published an article.Reference of N-Methyl-7H-purin-6-amine The title of the article was Structural insights into target DNA recognition by R2R3-MYB transcription factors. And the article contained the following:
As the largest group of MYB family transcription factors, R2R3-MYB proteins play essential roles during plant growth and development. However, the structural basis underlying how R2R3-MYBs recognize the target DNA remains elusive. Here, we report the crystal structure of Arabidopsis WEREWOLF (WER), an R2R3-MYB protein, in complex with its target DNA. Structural anal. showed that the third α-helixes in both the R2 and R3 repeats of WER fit in the major groove of the DNA, specifically recognizing the DNA motif 5′-AACNGC-3′. In combination with mutagenesis, in vitro binding and in vivo luciferase assays, we showed that K55, N106, K109 and N110 are critical for the function of WER. Although L59 of WER is not involved in DNA binding in the structure, ITC anal. suggested that L59 plays an important role in sensing DNA methylation at the fifth position of cytosine (5mC). Like 5mC, methylation at the sixth position of adenine (6mA) in the AAC element also inhibits the interaction between WER and its target DNA. Our study not only unravels the mol. basis of how WER recognizes its target DNA, but also suggests that 5mC and 6mA modifications may block the interaction between R2R3-MYB transcription factors and their target genes. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Reference of N-Methyl-7H-purin-6-amine
The Article related to dna recognition crystal structure r2r3 myb transcription factor arabidopsis, Biochemical Genetics: Gene Structure and Organization and other aspects.Reference of N-Methyl-7H-purin-6-amine
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem