On December 31, 2022, Liu, Chang; Li, Linshuang; Yang, Bo; Zhao, Yiqing; Dong, Xiyuan; Zhu, Lixia; Ren, Xinling; Huang, Bo; Yue, Jing; Jin, Lei; Zhang, Hanwang; Wang, Lan published an article.Application of 443-72-1 The title of the article was Transcriptome-wide N6-methyladenine methylation in granulosa cells of women with decreased ovarian reserve. And the article contained the following:
The emerging epitranscriptome plays an essential role in female fertility. As the most prevalent internal mRNA modification, N6-methyladenine (m6A) methylation regulate mRNA fate and translational efficiency. However, whether m6A methylation was involved in the aging-related ovarian reserve decline has not been investigated. Herein, we performed m6A transcriptome-wide profiling in the ovarian granulosa cells of younger women (younger group) and older women (older group). M6A methylation distribution was highly conserved and enriched in the CDS and 3’UTR region. Besides, an increased number of m6A methylated genes were identified in the older group. Bioinformatics anal. indicated that m6A methylated genes were enriched in the FoxO signaling pathway, adherens junction, and regulation of actin cytoskeleton. A total of 435 genes were differently expressed in the older group, moreover, 58 of them were modified by m6A. Several specific genes, including BUB1B, PHC2, TOP2A, DDR2, KLF13, and RYR2 which were differently expressed and modified by m6A, were validated using qRT-PCR and might be involved in the decreased ovarian functions in the aging ovary. Hence, our finding revealed the transcriptional significance of m6A modifications and provide potential therapeutic targets to promote fertility reservation for aging women. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Application of 443-72-1
The Article related to human ovarian reserve methyladenine methylation transcriptome granulosa cell, aging, granulosa cell, n6-methyladenosine, ovary, rna modification, Mammalian Pathological Biochemistry: Obstetrics – Gynecology and other aspects.Application of 443-72-1
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