On January 31, 2021, Joshi, Suraj; Ujaoney, Aman Kumar; Ghosh, Payel; Deobagkar, Deepti D.; Basu, Bhakti published an article.Name: N-Methyl-7H-purin-6-amine The title of the article was N6-methyladenine and epigenetic immunity of Deinococcus radiodurans. And the article contained the following:
DNA methylation is ubiquitously found in all three domains of life. This epigenetic modification on adenine or cytosine residues serves to regulate gene expression or to defend against invading DNA in bacteria. Here, we report the significance of N6-methyladenine (6mA) to epigenetic immunity in Deinococcus radiodurans. Putative protein encoded by DR_2267 ORF (Dam2DR) contributed 35% of genomic 6mA in D. radiodurans but did not influence gene expression or radiation resistance. Dam2DR was characterized to be a functional S-adenosyl methionine (SAM)-dependent N6-adenine DNA methyltransferase (MTase) but with no endonuclease activity. Adenine methylation from Dam2DR or Dam1DR (N6-adenine MTase encoded by DR_0643) improved DNA uptake during natural transformation. To the contrary, methylation from Escherichia coli N6-adenine MTase (DamEC that methylates adenine in GATC sequence) on donor plasmid drastically reduced DNA uptake in D. radiodurans, even in presence of Dam2DR or Dam1DR methylated adenines. With these results, we conclude that self-type N6-adenine methylation on donor DNA had a protective effect in absence of addnl. foreign methylation, a sep. methylation-dependent Restriction Modification (R-M) system effectively identifies and limits uptake of G6mATC sequence containing donor DNA. This is the first report demonstrating presence of epigenetic immunity in D. radiodurans. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Name: N-Methyl-7H-purin-6-amine
The Article related to deinococcus n6 methyladenine epigenetic immunity, dna uptake, dr_2267, deinococcus radiodurans, n6-methyladenine, restriction modification, transformation efficiency, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.Name: N-Methyl-7H-purin-6-amine
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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem