On March 15, 2022, Iwasaki, Yuka; Ookuro, Yurino; Iida, Keisuke; Nagasawa, Kazuo; Yoshida, Wataru published an article.Synthetic Route of 443-72-1 The title of the article was Destabilization of DNA and RNA G-quadruplex structures formed by GGA repeat due to N6-methyladenine modification. And the article contained the following:
N6-methyladenine (m6A) is the most abundant RNA modification in eukaryotic RNA. Further, m6A has been identified in the genomic DNA of both eukaryotes and prokaryotes. The G-quadruplex (G4) structure is a non-canonical nucleic acid structure formed by the stacking of G:G:G:G tetrads. In this study, we evaluated the effect of m6A modifications on G4 structures formed by GGA repeat oligonucleotides, d(GGA)8, d(GGA)4, and r(GGA)4. The d(GGA)8 forms an intramol. tetrad:heptad:heptad:tetrad G4 structure, while d(GGA)4 forms a dimerized intermol. tetrad:heptad:heptad:tetrad G4 structure. r(GGA)4 forms a dimerized intermol. tetrad:hexad:hexad:tetrad G4 structure. CD melting anal. demonstrated that (1) m6A modifications destabilized the G4 structure formed by d(GGA)8, (2) m6A modification at A3 disrupted the G4 structure formed by d(GGA)4, and (3) m6A modification at A3 destabilized the G4 structure formed by r(GGA)4. M6A modifications may be involved in controlling G4 structure formation to regulate biol. functions. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Synthetic Route of 443-72-1
The Article related to dna rna gquadruplex structure destabilization gga repeat methyladenine modification, g-quadruplex, gga repeat, n(6)-methyladenine, thermal stability, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Synthetic Route of 443-72-1
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem