On March 15, 2021, Diao, Li-Ting; Xie, Shu-Juan; Yu, Pei-Jie; Sun, Yu-Jia; Yang, Fan; Tan, Ye-Ya; Tao, Shuang; Hou, Ya-Rui; Zheng, Ling-Ling; Xiao, Zhen-Dong; Zhang, Qi published an article.Formula: C6H7N5 The title of the article was N6-methyladenine demethylase ALKBH1 inhibits the differentiation of skeletal muscle. And the article contained the following:
DNA N6-methyladenine (N6-mA) was recently recognized as a new epigenetic modification in mammalian genome, and ALKBH1 was discovered as its demethylase. Knock-out mice studies revealed that ALKBH1 was indispensable for normal embryonic development. However, the function of ALKBH1 in myogenesis is largely unknown. In this study, we found that N6-mA showed a steady increase, going along with a strong decrease of ALKBH1 during skeletal muscle development. Our results also showed that ALKBH1 enhanced proliferation and inhibited differentiation of C2C12 cells. Genome-wide transcriptome anal. and reporter assays further revealed that ALKBH1 accomplished the differentiation inhibiting function by regulating a core set of genes and multiple signaling pathways, including increasing chemokine (C-X-C motif) ligand 14 (CXCL14) and activating ERK signaling. Taken together, our results demonstrated that ALKBH1 is critical for the myogenic differentiation of C2C12 cells, and suggested that N6-mA might be a new epigenetic mechanism for the regulation of myogenesis. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).Formula: C6H7N5
The Article related to n6methyladenine dna methylation alkbh1 skeletal muscle differentiation, alkbh1, n(6)-methyladenine, rna-seq, skeletal muscle differentiation, Mammalian Biochemistry: General Physiological Chemistry and other aspects.Formula: C6H7N5
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem