On July 15, 2022, Zhu, Jun; Geng, Qiang; Liu, Yuan-Yang; Pan, Jiang; Yu, Hui Lei; Xu, Jian-He published an article.SDS of cas: 73590-85-9 The title of the article was Co-Cross-Linked Aggregates of Baeyer-Villiger Monooxygenases and Formate Dehydrogenase for Repeated Use in Asymmetric Biooxidation. And the article contained the following:
Baeyer-Villiger monooxygenases (BVMOs) are versatile biocatalysts, but their applications are hindered by their poor stability and cofactor dependence. In this study, cross-linked enzyme aggregate (CLEA) technol. was adopted to coimmobilize BVMO and its accessory cofactor-regeneration enzyme. Combi-CLEAs of a pyrmetazole monooxygenase from Acinetobacter calcoaceticus (AcPSMO) and a formate dehydrogenase from Burkholderia stabili (BstFDH) were prepared for the synthesis of (S)-omeprazole. After optimization, AcPSMO and BstFDH were coprecipitated with an activity ratio of 1:6 using ammonium sulfate and then cross-linked with glutaraldehyde (0.12% w/v). The activity recoveries of AcPSMO and BstFDH in the prepared combi-CLEAs were 43% and 38%, resp. Compared with the free enzymes AcPSMO and BstFDH, the thermostabilities of AcPSMO and BstFDH in combi-CLEAs were improved by 2.5- and 1.6-fold, resp. Both enzymes were more stable against alk. buffer after being immobilized. The combi-CLEAs could be reused for seven cycles in the biooxidative synthesis of (S)-omeprazole without significant activity loss, indicating the excellent operational stability and reusability in repeated reactions for the enzymic synthesis of (S)-omeprazole. Another two combi-CLEAs prepared under the same conditions, TmCHMO-BstFDH and RpBVMO-BstFDH, can be reused for at least 15 consecutive batches for the cyclohexanone mono-oxygenation reaction, which indicates the promising potential for coimmobilization of BVMOs and FDH with CLEA methodol. The experimental process involved the reaction of 5-Methoxy-2-[[(4-methoxy-3,5-dimethyl-2-pyridyl)methyl]thio]benzimidazole(cas: 73590-85-9).SDS of cas: 73590-85-9
The Article related to baeyer villiger monooxygenase formate dehydrogenase crosslinked aggregate asym biooxidation, Fermentation and Bioindustrial Chemistry: Methods (Including Analysis) and other aspects.SDS of cas: 73590-85-9
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem