On February 7, 2020, Thelen, Adam Z.; O’Brien, Patrick J. published an article.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one The title of the article was Recognition of 1,N2-ethenoguanine by alkyladenine DNA glycosylase is restricted by a conserved active-site residue. And the article contained the following:
The adenine, cytosine, and guanine bases of DNA are susceptible to alkylation by the aldehyde products of lipid peroxidation and by the metabolic byproducts of vinyl chloride pollutants. The resulting adducts spontaneously cyclize to form harmful etheno lesions. Cells employ a variety of DNA repair pathways to protect themselves from these pro-mutagenic modifications. Human alkyladenine DNA glycosylase (AAG) is thought to initiate base excision repair of both 1,N6-ethenoadenine (εA) and 1,N2-ethenoguanine (εG). However, it is not clear how AAG might accommodate εG in an active site that is complementary to εA. This prompted a thorough investigation of AAG-catalyzed excision of εG from several relevant contexts. Using single-turnover and multiple-turnover kinetic analyses, we found that εG in its natural εG·εC context is very poorly recognized relative to εA·εT. Bulged and mispaired εG contexts, which can form during DNA replication, were similarly poor substrates for AAG. Furthermore, AAG could not recognize an εG site in competition with excess undamaged DNA sites. Guided by previous structural studies, we hypothesized that Asn-169, a conserved residue in the AAG active-site pocket, contributes to discrimination against εG. Consistent with this model, the N169S variant of AAG was 7-fold more active for excision of εG compared with the wildtype (WT) enzyme. Taken together, these findings suggest that εG is not a primary substrate of AAG, and that current models for etheno lesion repair in humans should be revised. We propose that other repair and tolerance mechanisms operate in the case of εG lesions. The experimental process involved the reaction of Imidazo[1,2-c]pyrimidin-5(6H)-one(cas: 55662-66-3).Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one
The Article related to human alkyladenine dna glycosylase active site recognition ethenoguanine repair, dna alkylation, dna damage, dna repair, alkyladenine dna glycosylase, base excision repair (ber), enzyme kinetics, ethenoguanine, substrate specificity and other aspects.Recommanded Product: Imidazo[1,2-c]pyrimidin-5(6H)-one
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Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem