On December 31, 2022, Takahashi, Yusuke; Shoura, Massa; Fire, Andrew; Morishita, Shinichi published an article.SDS of cas: 443-72-1 The title of the article was Context-dependent DNA polymerization effects can masquerade as DNA modification signals. And the article contained the following:
Single mol. measurements of DNA polymerization kinetics provide a sensitive means to detect both secondary structures in DNA and deviations from primary chem. structure as a result of modified bases. In one approach to such anal., deviations can be inferred by monitoring the behavior of DNA polymerase using single-mol., real-time sequencing with zero-mode waveguide. This approach uses a Single Mol. Real Time (SMRT)-sequencing measurement of time between fluorescence pulse signals from consecutive nucleosides incorporated during DNA replication, called the interpulse duration (IPD). In this paper we present an anal. of loci with high IPDs in two genomes, a bacterial genome (E. coli) and a eukaryotic genome (C. elegans). To distinguish the potential effects of DNA modification on DNA polymerization speed, we paired an anal. of native genomic DNA with whole-genome amplified (WGA) material in which DNA modifications were effectively removed. Adenine modification sites for E. coli are known and we observed the expected IPD shifts at these sites in the native but not WGA samples. For C. elegans, such differences were not observed Instead, we found a number of novel sequence contexts where IPDs were raised relative to the average IPDs for each of the four nucleotides, but for which the raised IPD was present in both native and WGA samples. The latter results argue strongly against DNA modification as the underlying driver for high IPD segments for C. elegans, and provide a framework for separating effects of DNA modification from context-dependent DNA polymerase kinetic patterns inherent in underlying DNA sequence for a complex eukaryotic genome. The experimental process involved the reaction of N-Methyl-7H-purin-6-amine(cas: 443-72-1).SDS of cas: 443-72-1
The Article related to dna polymerization effect masquerade modification signal, dna n6-methyladenine, dna modification, dna polymerization, non-b dna, single-molecule real-time (smrt) sequencing, whole genome amplification and other aspects.SDS of cas: 443-72-1
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem