《Improved Coverage of Mouse Myelomeningocele With a Mussel Inspired Reverse Thermal Gel》 was written by Bardill, James R.; Park, Daewon; Marwan, Ahmed I.. Safety of Di(1H-imidazol-1-yl)methanone And the article was included in Journal of Surgical Research in 2020. The article conveys some information:
Myelomeningocele (MMC) is an open neural tube defect of the spinal column. Our laboratory previously introduced a reverse thermal gel (RTG) as the first in situ forming patch for in utero MMC application. To overcome the challenges of anchoring the RTG in the wet amniotic environment to improve MMC coverage, we modified the RTG to mimic the underwater adhesive properties of mussels. We have separated this study into three sep. hypotheses-based components:Based on mussel inspired chem., modification of the RTG with dopamine will increase the underwater adhesive properties to improve RTG anchoring ability in a wet environment. Methods: The dopamine-modified RTG (DRTG) was synthesized using carbonyldiimidazole chem. and characterized with proton NMR, Fourier transform IR spectroscopy, and UV-visible spectroscopy. Rheol. and underwater adhesive tests measured mech. properties. Results: DRTG synthesis was confirmed with proton NMR, Fourier transform IR spectroscopy, and UV-visible spectroscopy. Rheol. demonstrated increased elasticity. Underwater adhesion testing revealed DRTG has similar wet adhesive strength to Tisseel fibrin sealant.The DRTG will support in vitro skin cell growth and will be safe for injection in a mouse animal model. Methods: Biocompatibility testing included 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, DRTG-fibroblast and keratinocyte cultures, and s.c. injections to quantify macrophages stained with immunohistochem. Results: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and DRTG cell cultures revealed no cytotoxicity and demonstrated the growth of fibroblasts and keratinocytes. S.c. injections cause a macrophage response that decreases after 4 wk.In utero injection of novel DRTG into a mouse MMC model will be effective with improved patch coverage of the MMC defect. Methods: MMC coverage by DRTG was assessed using in utero mouse injections in the Grainy head-like 3 (Grhl3) mouse model. Results: In utero Grhl3 mouse injections demonstrate statistically significant (P = 0.012) improved MMC coverage of DRTG compared with previous RTG coverage with no significant macrophage response.The DRTG demonstrates increased elasticity, cellular scaffolding properties, and improved MMC coverage in the Grhl3 mouse model. Future studies will be translated to the preclin. ovine model to evaluate this novel gel. In addition to this study using Di(1H-imidazol-1-yl)methanone, there are many other studies that have used Di(1H-imidazol-1-yl)methanone(cas: 530-62-1Safety of Di(1H-imidazol-1-yl)methanone) was used in this study.
Di(1H-imidazol-1-yl)methanone(cas: 530-62-1) is a coupling agent in the synthesis of dipolar polyamides for nonlinear optical applications and polypeptides. It also used to make β-keto sulfones and sulfoxides, lead sequestering agents, and β-enamino acid derivatives.Safety of Di(1H-imidazol-1-yl)methanone
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem