Zhao, Xiaojia; Zhu, Zicheng; Zou, Rong; Wang, Lingyun; Gong, Hang; Cai, Changqun published the artcile< An enzyme-free three-dimensional DNA walker powered by catalytic hairpin assembly for H5N1 DNA ratiometric detection>, Computed Properties of 452-06-2, the main research area is DNA walker catalytic hairpin assembly ratiometric detection.
The reliable and quant. detection of virus DNA is highly desirable for the early diagnosis and clin. treatment of diseases. In this work, a three-dimensional (3D) DNA walker based on a non-enzyme-mediated nucleic acid cascade amplification reaction was constructed for the ratiometric detection of H5N1 DNA. When the DNA walker was developed by the surface modification of aminated silica microsphere (SiO2-NH2) with H1 hairpin structures were activated in the presence of target DNA, the fluorescence of the H1-conjugated Thioflavin T (ThT) fluorophore (495 nm) was turned on, while the fluorescence of 2-aminopurine (2-AP) (363 nm) conjugated to the H2 hairpin remained unchanged. This strategy combines DNA walker powered by catalyzed hairpin assembly (CHA) reaction with proportional fluorescence signal output methods, which can effectively reduce the risk of generating false-pos. signals. The developed DNA walker demonstrated excellent anal. sensitivity and specificity, with a detection limit of 60 pM. The DNA walker was also capable of selectively detecting H5N1 DNA in clin. blood serum samples, which demonstrated its potential for use in various clin. applications.
Microchemical Journal published new progress about Blood serum. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Computed Properties of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem