Zhao, Han; Chen, Mingjian; Ma, Changbei published the artcile< Fluorescent method for the detection of biothiols using an Ag+-mediated conformational switch>, COA of Formula: C5H5N5, the main research area is silver glutathione cysteine biothiol fluorescence conformational switch; 2-aminopurine; cysteine; fluorescence; glutathione.
In this work, a novel, simple, and time-saving fluorescence approach for the detection of biothiols (glutathione and cysteine) was developed by employing a DNA probe labeled with 2-aminopurine. As an adenine analog, 2-aminopurine exhibits high fluorescence intensity that can be rapidly quenched in the presence of DNA. In the presence of Ag+, the fluorescence increased significantly, which was a result of the formation of cytosine-Ag+-cytosine base pairs and the release of 2-aminopurine. Upon addition of either glutathione or cysteine, the structure of cytosine-Ag+-cytosine was disrupted, a product of the stronger affinity between biothiols and Ag+. As a result, the 2-aminopurine-labeled DNA probe returned to its former structure, and the fluorescence signal was quenched accordingly. The detection limit for glutathione and cysteine was 3 nM and 5 nM, resp. Furthermore, the determination of biothiols in human blood serum provided a potential application for the probe as a diagnostic tool in clin. practice.
Sensors published new progress about Blood serum. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, COA of Formula: C5H5N5.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem