Formula: C10H16N2O3SIn 2020 ,《Variability in Streptavidin-Sepharose Matrix Quality Can Significantly Affect Proximity-Dependent Biotinylation (BioID) Data》 was published in Journal of Proteome Research. The article was written by St-Germain, Jonathan R.; Samavarchi Tehrani, Payman; Wong, Cassandra; Larsen, Brett; Gingras, Anne-Claude; Raught, Brian. The article contains the following contents:
Due to their ease of use and high binding affinity, streptavidin-based purification tools have become widely used for isolating biotinylated compounds from complex mixtures The authors and others routinely use streptavidin-sepharose matrixes to isolate biotinylated polypeptides generated in proximity-dependent biotinylation approaches, such as BioID or APEX. However, the authors noted sporadic, substantial variation in the quality of BioID experiments performed in the same laboratories over time, using seemingly identical protocols. Identifying the source of this problem, here, the authors highlight considerable variability in streptavidin contamination derived from different production lots of streptavidin-sepharose beads from the same manufacturer and demonstrate that high levels of streptavidin peptide contamination can have detrimental effects on BioID data. The authors also describe two simple, rapid approaches to assess the degree of streptavidin “”shedding”” from individual lots of the sepharose matrix before use to avoid the use of lower quality reagent. The experimental part of the paper was very detailed, including the reaction process of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Formula: C10H16N2O3S)
5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Formula: C10H16N2O3S And it has been used as a vitamin supplement for the growth of Bacillus species.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem