《Effects of primary amine-based coatings on microglia internalization of nanogels》 was published in Colloids and Surfaces, B: Biointerfaces in 2020. These research results belong to Mauri, Emanuele; Veglianese, Pietro; Papa, Simonetta; Rossetti, Arianna; De Paola, Massimiliano; Mariani, Alessandro; Posel, Zbysek; Posocco, Paola; Sacchetti, Alessandro; Rossi, Filippo. Formula: C7H6N4O The article mentions the following:
Nanogels represent a pivotal class of biomaterials in the therapeutic intracellular treatment of many diseases, especially those involving the central nervous system (CNS). Their biocompatibility and synergy with the biol. environment encourage their cellular uptake, releasing the curative cargo in the desired area. As a main drawback, microglia are generally able to phagocytize any foreign element overcoming the blood brain barrier (BBB), including these materials, drastically limiting their bioavailability for the target cells. In this work, we investigated the opportunity to tune and therefore reduce nanogel internalization in microglia cultures, exploiting the orthogonal chem. functionalization with primary amine groups, as a surface coating strategy. Nanogels are designed by following two methods, the direct grafting of aliphatic primary amines and the linkage of -NH2 modified PEG on the nanogel surface. The latter synthesis was proposed to evaluate the combination of PEGylation with the basic nitrogen atom. The achieved results indicate the possibility of effectively modulating the uptake of nanogels, in particular limiting their internalization using the PEG-NH2 coating. This outcome could be considered a promising strategy for the development of carriers for drugs or gene delivery that could overcome microglia scavenging. In the experiment, the researchers used Di(1H-imidazol-1-yl)methanone(cas: 530-62-1Formula: C7H6N4O)
Di(1H-imidazol-1-yl)methanone(cas: 530-62-1) is a peptide coupling reagent,it is used in the synthesis of peptides. Reacts readily with carboxylic acids to form acyl imidazoles; subsequent reaction with amines to form amides goes smoothly.Formula: C7H6N4O
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem