Recommanded Product: 58-85-5In 2020 ,《Biotin synthesis in Ralstonia eutropha H16 utilizes pimeloyl coenzyme A and can be regulated by the amount of acceptor protein》 appeared in Applied and Environmental Microbiology. The author of the article were Eggers, Jessica; Strittmatter, Carl Simon; Kuesters, Kira; Biller, Emre; Steinbuechel, Alexander. The article conveys some information:
The biotin metabolism of the Gram-neg. facultative chemolithoautotrophic bacterium Ralstonia eutropha (syn. Cupriavidus necator), which is used for biopolymer production in industry, was investigated. A biotin auxotroph mutant lacking bioF was generated, and biotin depletion in the cells and the minimal biotin demand of a biotin-auxotrophic R. eutropha strain were determined Three consecutive cultivations in biotin-free medium were necessary to prevent growth of the auxotrophic mutant, and 40 ng/mL biotin was sufficient to promote cell growth. Nevertheless, 200 ng/mL biotin was necessary to ensure growth comparable to that of the wild type, which is similar to the demand of biotin-auxotrophic mutants among other prokaryotic and eukaryotic microbes. A phenotypic complementation of the R. eutropha ΔbioF mutant was only achieved by homologous expression of bioF of R. eutropha or heterologous expression of bioF of Bacillus subtilis but not by bioF of Escherichia coli. Together with the results from bioinformatic anal. of BioFs, this leads to the assumption that the intermediate of biotin synthesis in R. eutropha is pimeloyl-CoA instead of pimeloyl-acyl carrier protein (ACP) like in the Gram-pos. B. subtilis. Internal biotin content was enhanced by homologous expression of accB, whereas homologous expression of accB and accC2 in combination led to decreased biotin concentrations in the cells. Although a DNA-binding domain of the regulator protein BirA is missing, biotin synthesis seemed to be influenced by the amount of acceptor protein present. The experimental part of the paper was very detailed, including the reaction process of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Recommanded Product: 58-85-5)
5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Recommanded Product: 58-85-5 And it has been used as a vitamin supplement for the growth of Bacillus species.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem