Tang, Jin; Tang, Feng; Zhao, Linlin published the artcile< Facile preparation of model DNA interstrand crosslink repair intermediates using ribonucleotide-containing DNA>, Electric Literature of 452-06-2, the main research area is DNA interstrand crosslink repair ribonucleotide; DNA damage; DNA interstrand cross-links; DNA repair; RNase H; Translesion synthesis (TLS).
DNA interstrand crosslinks (ICLs) are lesions with a covalent bond formed between DNA strands. ICLs are extremely toxic to cells because they prevent the separation of the two strands, which are necessary for the genetic interpretation of DNA. ICLs are repaired via Fanconi anemia and replication-independent pathways. The formation of so-called unhooked repair intermediates via a dual strand incision flanking the ICL site on one strand is an essential step in nearly all ICL repair pathways. Recently, ICLs derived from endogenous sources, such as those from ubiquitous DNA lesions, abasic (AP) sites, have emerged as an important class of ICLs. Despite the earlier efforts in preparing AP-ICLs in high yield using nucleotide analogs, little information is available for preparing AP-ICL unhooked intermediates with varying lengths of overhangs. In this study, we devise a simple approach to prepare model ICL unhooked intermediates derived from AP sites. We exploited the alk. lability of ribonucleotides (rNMPs) and the high crosslinking efficiency between an AP lesion and a nucleotide analog, 2-aminopurine, via reductive amination. We designed chimeric DNA/RNA substrates with rNMPs flanking the crosslinking residue (2-aminopurine) to facilitate subsequent strand cleavage under our optimized conditions. Mass spectrometric anal. and primer extension assays confirmed the structures of ICL substrates. The method is straightforward, requires no synthetic chem. expertise, and should be broadly accessible to all researchers in the DNA repair community. For step-by-step descriptions of the method, please refer to the companion manuscript in MethodsX.
DNA Repair published new progress about Amination. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Electric Literature of 452-06-2.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem