Zhou, Huan; Li, Xuan; Yin, Yuan; He, Xiao-Tao; An, Ying; Tian, Bei-Min; Hong, Yong-Long; Wu, Li-An; Chen, Fa-Ming published the artcile< The proangiogenic effects of extracellular vesicles secreted by dental pulp stem cells derived from periodontally compromised teeth>, Application In Synthesis of 6823-69-4, the main research area is GW4869 pluripotent angiogenesis; Angiogenesis; Dental pulp stem cells; Extracellular vesicles; Inflammation; Periodontitis.
In this study, we investigated the proangiogenic effects of extracellular vesicles (EVs) secreted by P-DPSCs using in vitro and in vivo testing models. Patient-matched DPSCs derived from periodontally healthy teeth (H-DPSCs) were used as the control for P-DPSCs. Conditioned media (CMs) derived from H-DPSCs and P-DPSCs (H-CM and P-CM), CMs derived from both cell types pretreated with the EV secretion blocker GW4869 (H-GW and P-GW), and EVs secreted by H-DPSCs and P-DPSCs (H-EVs and P-EVs) were prepared to test their proangiogenic effects on endothelial cells (ECs). Cell proliferation, migration, and tube formation were assessed using the Cell Counting Kit-8 (CCK-8), transwell/scratch wound healing, and Matrigel assays, resp. Finally, a full-thickness skin defect model was applied to test the effects of EVs on wound healing and new vessel formation. Both H-CM and P-CM promoted EC angiogenesis, but the proangiogenic effects were compromised when ECs were incubated in H-GW and P-GW, wherein the EV secretion was blocked by pretreatment with GW4869. In EV-based incubations, although both H-EVs and P-EVs were found to enhance the angiogenesis-related activities of ECs, P-EVs exerted a more robust potential to stimulate EC proliferation, migration, and tube formation. The findings of the present study provide addnl. evidence that P-DPSCs derived from periodontally diseased teeth represent a potential source of cells for research and therapeutic use.
Stem Cell Research & Therapy published new progress about Angiogenesis. 6823-69-4 belongs to class imidazoles-derivatives, and the molecular formula is C30H30Cl2N6O2, Application In Synthesis of 6823-69-4.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem