Mashiach, Daniel; Bacasen, Erin Mae; Singh, Sunjum; Kao, Timothy; Yaramada, Lekha; Mishail, Daniel; Singh, Summer; Miller, Jeffrey H. published the artcile< Enhanced characterization of the thyA system for mutational analysis in Escherichia coli: Defining mutationally ""hot"" regions of the gene>, Computed Properties of 452-06-2, the main research area is Escherichia thyA system mutation; Catalog; Cisplatin; Hotspots; Mutation; thyA system.
We have extensively characterized base substitution mutations in the 795 base pair (bp) long E. coli thyA gene to define as many of the base substitution mutational sites that inactivate the gene as possible. The resulting catalog of mutational sites constitutes a system with up to 5 times as many sites for monitoring each of the six base substitution mutations as the widely used rpoB/Rifr system. We have defined 75 sites for the G:C -> A:T transition, 68 sites for the G:C -> T:A transversion, 53 sites for the G:C -> C:G transversion, 49 sites for the A:T -> G:C transition, 39 sites for the A:T -> T:A transversion, and 59 sites for the A:T -> C:G transversion. This allows for the examination of mutational spectra using a more detailed probe of known mutations, while still allowing one to compare the number of repeated occurrences at specific sites. We have examined several mutagens and mutators with this system, and show its utility by looking at the spectrum of cisplatin, that has a single hotspot, underscoring the value of having as large an array of sites as possible at which one can monitor repeat occurrences. The resulting graphs suggest that there are “”hot”” regions at intervals, and this may reflect aspects of secondary structures, of the higher order structure of the chromosome, or perhaps the nucleoid structure of the chromosome plus histone-like protein complexes.
Mutation Research, Fundamental and Molecular Mechanisms of Mutagenesis published new progress about Chromosome. 452-06-2 belongs to class imidazoles-derivatives, and the molecular formula is C5H5N5, Computed Properties of 452-06-2.
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Imidazole | C3H4N2 – PubChem