The author of 《The First MS-Cleavable, Photo-Thiol-Reactive Cross-Linker for Protein Structural Studies》 were Iacobucci, Claudio; Piotrowski, Christine; Rehkamp, Anne; Ihling, Christian H.; Sinz, Andrea. And the article was published in Journal of the American Society for Mass Spectrometry in 2019. Application In Synthesis of Di(1H-imidazol-1-yl)methanone The author mentioned the following in the article:
Cleavable crosslinkers are gaining increasing importance for chem. crosslinking/mass spectrometry (MS) as they permit a reliable and automated data anal. in structural studies of proteins and protein assemblies. Here, the authors introduce 1,3-diallylurea (DAU) as the first CID-MS/MS-cleavable, photo-thiol-reactive crosslinker. DAU is a com. available, inexpensive reagent that efficiently undergoes an anti-Markovnikov hydrothiolation with cysteine residues in the presence of a radical initiator upon UV-A irradiation Radical cysteine crosslinking proceeds via an orthogonal “”click reaction”” and yields stable alkyl sulfide products. DAU reacts at physiol. pH and crosslinking reactions with peptides, and proteins can be performed at temperatures as low as 4°. The central urea bond is efficiently cleaved upon collisional activation during tandem MS experiments generating characteristic product ions. This improves the reliability of automated crosslink identification. Different radical initiators have been screened for the crosslinking reaction of DAU using the thiol-containing compounds cysteine and glutathione. The authors’ concept has also been exemplified for the biol. relevant proteins bMunc13-2 and retinal guanylyl cyclase-activating protein-2. After reading the article, we found that the author used Di(1H-imidazol-1-yl)methanone(cas: 530-62-1Application In Synthesis of Di(1H-imidazol-1-yl)methanone)
Di(1H-imidazol-1-yl)methanone(cas: 530-62-1) is a coupling agent in the synthesis of dipolar polyamides for nonlinear optical applications and polypeptides. It also used to make β-keto sulfones and sulfoxides, lead sequestering agents, and β-enamino acid derivatives.Application In Synthesis of Di(1H-imidazol-1-yl)methanone
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem