《Split-TurboID enables contact-dependent proximity labeling in cells》 was published in Proceedings of the National Academy of Sciences of the United States of America in 2020. These research results belong to Cho, Kelvin F.; Branon, Tess C.; Rajeev, Sanjana; Svinkina, Tanya; Udeshi, Namrata D.; Thoudam, Themis; Kwak, Chulhwan; Rhee, Hyun-Woo; Lee, In-Kyu; Carr, Steven A.; Ting, Alice Y.. Application In Synthesis of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid The article mentions the following:
Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the proteomic anal. of subcellular regions difficult or impossible to access by conventional fractionation-based approaches. Yet some cellular regions, such as organelle contact sites, remain out of reach for current PL methods. To address this limitation, we split the enzyme TurboID into two inactive fragments that recombine when driven together by a protein-protein interaction or membrane-membrane apposition. At endoplasmic reticulum-mitochondria contact sites, reconstituted TurboID catalyzed spatially restricted biotinylation, enabling the enrichment and identification of >100 endogenous proteins, including many not previously linked to endoplasmic reticulum-mitochondria contacts. We validated eight candidates by biochem. fractionation and overexpression imaging. Overall, split-TurboID is a versatile tool for conditional and spatially specific proximity labeling in cells. The experimental process involved the reaction of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5Application In Synthesis of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid)
5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.Application In Synthesis of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid And it has been used for blocking endogenous biotin during immunohistology procedures.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem