The author of 《Avoiding Pre-Isolation Step in Exosome Analysis: Direct Isolation and Sensitive Detection of Exosomes Using Gold-Loaded Nanoporous Ferric Oxide Nanozymes》 were Boriachek, Kseniia; Masud, Mostafa Kamal; Palma, Carlos; Phan, Hoang-Phuong; Yamauchi, Yusuke; Hossain, Shahriar A. Md.; Nguyen, Nam-Trung; Salomon, Carlos; Shiddiky, Muhammad J. A.. And the article was published in Analytical Chemistry (Washington, DC, United States) in 2019. COA of Formula: C10H16N2O3S The author mentioned the following in the article:
Most of the current exosome-anal. strategies are time-consuming and largely dependent on com. extraction kit-based preisolation step, which requires extensive sample manipulations, costly isolation kits, reagents, tedious procedures, and sophisticated equipment and is prone to bias/artifacts. Herein we introduce a simple method for direct isolation and subsequent detection of a specific population of exosomes using an engineered superparamagnetic material with multifunctional properties, namely, gold-loaded ferric oxide nanocubes (Au-NPFe2O3NC). In this method, the Au-NPFe2O3NC were initially functionalized with a generic tetraspanin (exosomes-associated) antibody (i.e., CD63) and dispersed in sample fluids where they work as “”dispersible nanocarriers”” to capture the bulk population of exosomes. After magnetic collection and purification, Au-NPFe2O3NC-bound exosomes were transferred to the tissue-specific, antibody-modified, screen-printed electrode. As a proof of principle, we used a specific placental marker, placenta alk. phosphatase (PLAP), to detect exosomes secreted from placental cells. The peroxidase-like activity of Au-NPFe2O3NC was then used to accomplish an ELISA-based sensing protocol for naked-eye observation along with UV-visible and electrochem. detection of PLAP-specific exosomes present in placental cell-conditioned media. We demonstrated excellent agreement in anal. performance for the detection of placental cell-derived exosomes (i.e., linear dynamic range, 103-107 exosomes/mL; limit of detection, 103 exosomes/mL; relative standard deviation (%RSD) of <5.5% for n = 3) using with and without com. ""total exosome isolation kit""-based preisolation step. We envisage that this highly sensitive, rapid, and inexpensive assay could be useful in quantifying specific populations of exosomes for various clin. applications, focusing on pregnancy complications. The experimental process involved the reaction of 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5COA of Formula: C10H16N2O3S)
5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoic acid(cas: 58-85-5) may be used to elute proteins from avidin/streptavidin resins. It has been used for culturing of oligodendrocytes.COA of Formula: C10H16N2O3S And it has been used for blocking endogenous biotin during immunohistology procedures.
Referemce:
Imidazole – Wikipedia,
Imidazole | C3H4N2 – PubChem